PENGGUNAAN ENZIM PEPSIN UNTUK PRODUKSI HIDROLISAT PROTEIN KACANG GUDE (Cajanus cajan (L.) Millsp.) YANG AKTIF ANTIOKSIDAN

  • Helen Helda Prastika Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia
  • Ketut Ratnayani Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia
  • Ni Made Puspawati Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia
  • A.A.I.A. Mayun Laksmiwati Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia

Abstract

ABSTRAK: Penelitian tentang penggunaan enzim pepsin untuk produksi hidrolisat protein kacang gude (Cajanus cajan (L.) Millsp.) yang aktif antioksidan telah dilakukan dengan variasi waktu hidrolisis 0, 30, 60, 90, dan 120 menit. Hidrolisis protein enzimatik merupakan metode yang sangat efisien untuk menghasilkan bioaktif peptida termasuk menunjukkan aktivitas antioksidan. Penelitian ini dilakukan untuk mengetahui konsentrasi asam amino bebas yang dilepaskan selama proses hidrolisis, nilai derajat hidrolisis, aktivitas antioksidan, dan pengaruh waktu hidrolisis terhadap aktivitas antioksidan. Konsentrasi asam amino diukur menggunakan standar asam amino leusin dengan menggunakan spektrofotometer pada ?maks 570 nm dan diperoleh konsentrasi tertinggi hidrolisat protein sebesar 2,4671 mg/mL. Enzim pepsin mampu menghidrolisis protein dengan nilai derajat hidrolisis tertinggi sebesar 23,59%. Aktivitas antioksidan diuji secara in vitro menggunakan radikal DPPH dan diukur pada ?maks 517 nm. Berdasarkan penelitian hidrolisat kacang gude mempunyai aktivitas antioksidan tertinggi sebesar 82,46%. Hasil penelitian menunjukkan adanya pengaruh variasi waktu hidrolisis terhadap terhadap peningkatan jumlah asam amino bebas, nilai derajat hidrolisis, dan aktivitas antioksidan. Diketahui bahwa semakin lama waktu hidrolisis, hasil yang diperoleh semakin meningkat. Hasil tertinggi pada masing-masing proses tersebut terjadi pada waktu 120 menit sehingga hasil dimungkinkan masih dapat meningkat dengan penambahan waktu hidrolisis.


Kata kunci: bioaktif peptida, kacang gude, pepsin, derajat hidrolisis, antioksidan


 


ABSTRACT: The study of the effect of pepsin enzyme for the production of pigeon pea (Cajanus cajan (L.) Millsp.) protein hydrolyzate which is active as antioxidants has been carried out with variations in hydrolysis time of 0, 30, 60, 90, and 120 minutes. Enzymatic protein hydrolysis is a very efficient method for producing bioactive peptides and also showing antioxidant activity. This study was conducted to determine the concentration of free amino acids released during the hydrolysis process, the value of hydrolysis degrees, antioxidant activity, and the influence of hydrolysis time on antioxidant activity. Amino acid concentrations were measured using standard amino acid leucine using a spectrophotometer at ?max 570 nm and obtained the highest concentration of protein hydrolyzate at 2,4671 mg / mL. The pepsin enzyme is able to hydrolyze proteins with the highest hydrolysis degree value of 23.59%. Antioxidant activity was tested in vitro using DPPH radical and measured at ?maks 517 nm. Based on the research of pigeon pea hydrolyzate it has the highest antioxidant activity of 82.46%. The results showed the influence of the variation of hydrolysis time on the increase in the number of free amino acids, the value of hydrolysis degrees, and antioxidant activity. It is known that the longer the hydrolysis time, the results obtained increase. The highest results in each of these processes occur at 120 minutes so that the results are possible can still increase with the addition of hydrolysis time.

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References

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Published
2019-10-31
How to Cite
PRASTIKA, Helen Helda et al. PENGGUNAAN ENZIM PEPSIN UNTUK PRODUKSI HIDROLISAT PROTEIN KACANG GUDE (Cajanus cajan (L.) Millsp.) YANG AKTIF ANTIOKSIDAN. CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry), [S.l.], v. 7, n. 2, p. 180-188, oct. 2019. ISSN 2302-7274. Available at: <https://ojs.unud.ac.id/index.php/cakra/article/view/56199>. Date accessed: 30 sep. 2022.