Study of Total Phenol, Flavonoids and Antioxidant Activity of Kidney Beans (Phaseolus Vulgaris L.) at Various Length of Germination Times

Abstract

The purpose of this study are 1) to find out whether the length of germination times affects on the total
phenol, flavonoids and antioxidant activity of kidney bean sprouts 2). To determine the total phenol,
flavonoids and antioxidant activity of kidney beans at various germination times 3). Determine the best
germination time and the nutrient composition. The germination time in this study were 0, 24, 36, 48,
60 and 72 hours. This study used a completely randomized design with three replications in order to
obtain 18 experimental units. Data analysis based on ANOVA, if the treatment affects the observed
varables then tested with Duncant. The results showed that the length of germination time very
significant effect on the total phenols, flavonoids and antioxidant activity of kidney bean sprouts. Total
phenol content of 4.46-6.33%, flavonoid levels of 1.17-9.23 mg/100 g QE, and antioxidant activity of
53.91-65.10 ppm (IC 50%). The best length of germination time was 48 hours with nutritional
composition: water content 64.83%, ash 0.94%, fat 3.69%, protein 25.61% and carbohydrate 4.93%.