Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES) | Jurnal Veteriner

PDF

Published Sep 17, 2013

I Wayan Suardana

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

I Nengah Sujaya Wayan Tunas Artama

Abstract

A Shiga-like toxin producing Escherichia coli O157:H7 was detected in faecal samples of cattle, andhuman as well as in beef. The performance of agent indicated that it has been identified as harmful andoften life-threatening zoonotic agent. It is therefore important to analysed the genetic characteristic ofShiga toxin Escherichia coli (STEC) and to develop a diagnostic probe in order to optimalized of diagnostictest for the agent. The study was started by amplifiying stx2 gene, purifying of PCR product, sequencingof stx2 gene, analyzing of phylogenetic tree, and finally by analyzing of diagnostic probe candidate.Homology study showed that the genetic sequence of the local isolate of E. coli O157:H7 i.e SM25(1)isolated from cattle feces has a genetic and fuctional similarity with the control isolate i.e E. coli O157:H7ATCC 43894 originated from human. Further study showed that a probe with foreward primer sequanceof 5’-AATTTATATGTGGCCGGGTTC-‘3 which were respectively designed as a PFS and PRS 176 bp product.Appeared to be potential candidate of diagnostic probe for the agent.

Downloads

Download data is not yet available.

How to Cite

SUARDANA, I Wayan; SUJAYA, I Nengah; ARTAMA, Wayan Tunas. Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES). Jurnal Veteriner, [S.l.], v. 14, n. 2, p. 153-163, sep. 2013. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/6430>. Date accessed: 18 nov. 2024.

ABNT APA BibTeX CBE EndNote - EndNote format (Macintosh & Windows) MLA ProCite - RIS format (Macintosh & Windows) RefWorks Reference Manager - RIS format (Windows only) Turabian

Keywords

E.coli O157:H7, molecular biology, probe, stx2 gene

Issue

Vol 14 No 2 (2013)

Section

Articles

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License

Most read articles by the same author(s)

Ida Ayu Pasti Apsari, Wayan Tunas Artama, Sumartono ., I Made Damriyasa, Diagnosis Molekuler Toxoplasma gondii Berdasar Gen Stage Spesifik Takizoit dan Bradizoit pada Ayam Kampung (MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII BASED ON THE TACHYZOITE AND BRADYZOITE STAGE SPECIFIC GENES IN FREE-RANGE CHICKEN) , Jurnal Veteriner: Vol 13 No 1 (2012)
I Wayan Suardana, I Nyoman Suarsana, I Nengah Sujaya, Komang Gede Wiryawan, Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali sebagai Kandidat Biopreservatif ISOLATION AND IDENTIFICATION OF ACID LACTIC BACTERIA FROM BALI CATTLE’S GASTRIC FLUID AS A POTENTIAL CANDIDATE OF BIOPRESERVATIVE , Jurnal Veteriner: Vol 8 No 4 (2007)
I Nengah Sujaya, I Made Utami Dwipayanti, Ni Luh Putu Suariani, Ni Putu Widarini, Komang Ayu Nocianitri, Ni Wayan Nursini, PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK , Jurnal Veteriner: Vol 9 No 1 (2008)
Sorta Basar Ida Simanjuntak, Sukarti Moeljopawiro, Wayan Tunas Artama, Subagus Wahyuono, Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit , Jurnal Veteriner: Vol 12 No 4 (2011)
I Gusti Ayu Kusuma Dewi, I Gusti Ngurah Agung Dewantara Putra, I Nengah Sujaya, PENGEMBANGAN STARTER DARI Lactobacillus spp. ISOLAT SUSU KUDA SUMBAWA UNTUK PEMBUATAN SUSU TERFERMENTASI , Jurnal Farmasi Udayana: Vol. 2, No. 1, Tahun 2013
Ida Ayu Pasti Apsari, Wayan Tunas Artama, Sumartono ., I Made Damriyasa, Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE) , Jurnal Veteriner: Vol 13 No 4 (2012)
I Wayan Suardana, I Nengah Sujaya, Wayan Tunas Artama, Aplikasi Kandidat Pemindai untuk Diagnosis Gen Shiga like toxin-2 dari Escherichia coli O157:H7 (PROBE APLICATION TO DIAGNOSTIC PROGRAME OF SHIGA LIKE TOXIN-2 (STX2) GEN FROM ESCHERICHIA COLI O157:H7) , Jurnal Veteriner: Vol 13 No 4 (2012)
Enny Yusuf Wachidah Yuniwarti, Widya Asmara, Wayan Tunas Artama, Charles Rangga Tabbu, Virgin Coconut Oil Meningkatkan Aktivitas Fagositosis Makrofag Ayam Pedaging Pascavaksinasi Flu Burung (VIRGIN COCONUT OIL INCREASES THE PHAGOCYTOSIS ACTIVITY OF MACROPHAGE OF BROILER CHICKEN FOLLOWING AVIAN INFLUENZA VACCINATION) , Jurnal Veteriner: Vol 14 No 2 (2013)
Dewa Ayu Agus Sri Laksemi, Wayan Tunas Artama, Mahardika Agus Wijayanti, Seroprevalensi yang Tinggi dan Faktor-Faktor Risiko Toksoplasmosis pada Darah Donor dan Wanita di Bali (HIGH SEROPREVALENCE AND RISK FACTORS OF TOKSOPLASMOSIS AMONG BLOOD DONORS AND WOMEN IN BALI) , Jurnal Veteriner: Vol 14 No 2 (2013)
Rini Widayanti, Trini Susmiati, Wayan Tunas Artama, Keragaman Genetik Gen NADH Dehydrogenase Subunit 6 pada Monyet Hantu (Tarsius Sp.) (GENETIC DIVERSITY STUDY ON NADH DEHYDROGENASE SUBUNIT 6 GENE OF TARSIUS SP.) , Jurnal Veteriner: Vol 14 No 2 (2013)

1 2 > >>