Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE) | Jurnal Veteriner

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Published Jul 21, 2013

Ida Ayu Pasti Apsari

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Wayan Tunas Artama Sumartono . I Made Damriyasa

Abstract

Sag1 and bag1 is a gene specific-stage for Toxoplasma gondii tachyzoite and bradyzoite. The purposeof this study was analyze the sequences of sag1 and bag1 tachyzoite genes of local of Toxoplasma gondiiisolate as deoxyribonucleic acid probe candidate. Tachyzoite of local of Toxoplasma gondii isolate used onthis study. Gene sag1 and bag1 gene of Toxoplasma gondii were amplified by PCR, and then sequenced. Theresults showed sag1 fragment gene contained 612 bp and bag 1 contained 470 bp in length. BLAST analysisof sag1 and bag1 gene fragments as probe candidate showed that high specific for Toxoplasma gondii andno significant cross-reaction fragment with host and other parasites. The sequences 136 bp and 98 bpfragments as DNA probe candidate of Toxoplasma gondii sag1 and bag1 respectively.

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APSARI, Ida Ayu Pasti et al. Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE). Jurnal Veteriner, [S.l.], v. 13, n. 4, p. 330-339, july 2013. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/6023>. Date accessed: 21 nov. 2024.

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Keywords

Sag1 gene, Bag1 gene, tachyzoite, Toxoplasma gondii, DNA probe

Issue

Vol 13 No 4 (2012)

Section

Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License

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