Pengujian Bakteri Potensial Pendegradasi Dibenzothiophene (DBT) yang diisolasi dari Tanah yang Terkontaminasi Minyak Bumi di Samboja

Abstract

This study aims  to determine the  dibenzothiophene (DBT)  degradation  level  of  four
potential sulfur aromatic degrading bacteria. Biodesulfurization activity test was done by using twophase system. First was water phase  which used mineral salt sulfur free (MSSF) medium and the
second phase was oil phase which use 200 ppm DBT in n-tetradecane. Isolates were incubated for
96 hours at 37
o
C  and the DBT residu oil phase was analyzed using  Gas Chromatography. The
experiment was repeated twice. This isolate wa able to degrade DBT in level of 80,82% after 96
hours incubation. The growth of the bacteria in water phase reached OD
660
1,169.

Keywords: Dibenzothipohene, Biodesulfurization, Bacteria Potential, SBJ 8