MICROBIOLOGICAL OF BALI BEEF MARINED WITH DIFFERENT CONCENTRATIONS OF MORINGA LEAF POWDER (Moringa oleifera Lamk)
Abstract
This study aimed to investigate the effect of marinating with Moringa leaf powder solution with different con- centrations on microbiological of Bali beef stored at room temperature for 12 hours. The experimental design used was a completely randomized design (CRD) with four treatments and four replications. Each replication consisted of 100 g of beef. The four treatments were: P0 (Bali beef marinated in 0% solution of Moringa leaf powder), P1 (0.2% w/v solution of Moringa leaf powder), P2 (0.4% w/v solution of Moringa leaf powder), P3 (0.6% w/v solution of Moringa leaf powder). The variables observed in this study were the Total Plate Count (TPC), Total Coliform and Escherichia coli, the pH value, and the water content of the meat. The results showed that the treatment of Moringa leaf powder had a significant effect (P<0.05) on microbiological of Bali beef. The conclusion of this study is that the marinated solution of Moringa leaf powder affects the microbiological quality of Bali beef, which reduces the Total Plate Count (TPC), but has no effect on total Coliform, Escherichia coli, water content, and pH levels. Total Plate Count, Coliform, Escherichia coli, and all treatments were above the SNI threshold. It means that Bali beef mari- nated with concentrations of 0% (P0), 0.2% (P1), 0.4% (P2), and 0.6% (P3) and stored at room temperature for 12 hours is not suitable for consumption.
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References
Bukar, A., A. Uba, dan T. Oyeyi. 2010. Antimicrobial profile of moringa oleifera Lamk. Extracts against some food-borne microorganisms. Bayero J. Pure Appl. Sci. 3(1):43-48.
Busani, M., P.M. Julius, dan M. Voster. 2012. Antimicro- bial activities of Moringa oleifera Lam leaf extracts. African J. Biotech. 11(11):2797-2802.
Fardiaz, S. 1989. Mikrobiologi Pangan. Departemen Pendidikan dan Kebudayaan Direktoran Jendral Pendidikan Tinggi Pusat Antar Universitas Pangan dan Gizi. Institut Pertanian Bogor. Bogor.
Hernando, D., D. Septinova, dan K. Adhianto. 2015. Ka- dar air dan total mikroba pada daging sapi di Tem- pat Pemotongan Hewan (TPH) Bandar Lampung. Jurnal Ilmiah Peternakan Terpadu. 3(1):61-67.
Mashau, M.E., K.E. Ramatsetse, dan S.E Ramashia. 2021. Effects of adding Moringa oleifera leaves powder on the nutritional properties, lipid oxidation and microbial growth in ground beef during cold storage. Appl. Sci. 11(7).
Meha, N.L.A., Y.T. Ina, dan A. Kaka. 2022. Konsentrasi asap cair kayu kesambi (Scleichera leosa) dan pengaruhnya terhadap fisiko kimiawi daging sapi. Jurnal Teknologi Pertanian. 11(1):10-22.
Nurwantoro, V.P. Bintoro, A.M. Legowo, dan A. Pur- nomoadi. 2013. Pengolahan daging dengan sistem
marinasi untuk meningkatkan keamanan pangan dan nilai tambah. The Working Garde Manger. 22(2):212-221.
Putri, A.M. dan P. Kurnia. 2018. Identifikasi keberadaan bakteri coliform dan total mikroba dalam es dung- dung di sekitar Kampus Universitas Muhammadi- yah Surakarta. Media Gizi Indonesia. 13(1):41-48.
Rahman, F.A., T. Haniastuti, dan T.W. Utami. 2017. Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668. Majalah Ke- dokteran Gigi Indonesia. 3(1):1-7.
Rahmawati, S., A.U. Farahdiba, O. Alfan, dan R.B. Adhly. 2018. Identifikasi total coliform, Escherichia coli dan Salmonella Spp. sebagai indikator sanitasi makanan kantin di lingkungan Kampus Terpadu Universitas Islam Indonesia. Jurnal Sains dan Teknologi Lingkungan. 10(2):101-114.
Sartika, R.A.D., Y.M. Indrawani, dan T. Sudiarti. 2005. Analisis mikrobiologi Escherichia coli O157:H7 pada hasil olahan hewan sapi dalam proses produk- sinya. Makara Kesehatan. 9(1):23-28.
Sriyani, N.L.P., A. Tirta, S.A. Lindawati, dan I.N.S Mi- wada. 2015. Kajian kualitas fisik daging kambing yang dipotong di RPH tradisional Kota Denpasar. Majalah Ilmiah Peternakan. 18(2):48-51.
[SNI] Standar Nasional Indonesia. 2008. Standar Nasio- nal Indonesia Mutu Karkas dan Daging Sapi. Sutrisna, R., C.N. Ekowati, dan E. Sinaga. 2015. Pengaruh
pH terhadap produksi antibakteri oleh bakteri asam laktat dari usus itik. Jurnal Penelitian Pertanian Terapan. 15(3):234-238.
Yunita, M., Y. Hendrawan, R. Yulianingsih, dan K. Kunci. 2015. Analisis kuantitatif mikrobiologi pada makan- an penerbangan (Aerofood ACS) Garuda Indonesia berdasarkan TPC (Total Plate Count) dengan meto- de pour plate. Jurnal Keteknikan Pertanian Tropis dan Biosistem. 3(3):237-248.
Waluyo, L. 2010. Teknik dan Metode Dasar dalam Mik- robiologi. Universitas Muhammadiyah Malang Press. Malang.