SENSITIVITY AND SPECIFICITY OF ELISA TEST USING 30 KDA RECOMBINANT ANTIGEN TO DETECT Toxoplasma gondii INFECTION IN PIG WITH MICE BIOASSAY AS A GOLD STANDARD Nyoman Adi Suratma1????, I Made Bakta2, I Made Damriyasa3

  • Nyoman Adi Suratma
  • I Made Bakta
  • I Made Damriyasa

Abstract

Toxoplasmosis is a zoonotic disease caused by the protozoa Toxoplasma gondii.Primary infection in pregnant women can cause abortion, neonatal death or abnormalityof fetus. Accurate diagnosis is needed to prevent infection especially related to thepresence of cyst in the tissuesThe aim of this research was to study sensitivity and specificity of ELISAmethod with 30 kDa protein antigen to detect T.gondii infection in pig with micebioassay as gold standard.. Samples were 171 pigs slaughtered at pig slaughter house inDarmasaba BadungThe result showed that sensitivity of ELISA method was 100% and 90,7% inspecificity.Research about sensitivity using ELISA test to predict cysts presence in tissuewere needed in the future.

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Author Biographies

Nyoman Adi Suratma
Program Study of Medical Science, School for Graduate Study, Udayana University
I Made Bakta
Dept. of Internal Medicine, Faculty of Medicine, Udayana University
I Made Damriyasa
Faculty of Veterinary Madicine, Udayana University
How to Cite
ADI SURATMA, Nyoman; BAKTA, I Made; DAMRIYASA, I Made. SENSITIVITY AND SPECIFICITY OF ELISA TEST USING 30 KDA RECOMBINANT ANTIGEN TO DETECT Toxoplasma gondii INFECTION IN PIG WITH MICE BIOASSAY AS A GOLD STANDARD Nyoman Adi Suratma1????, I Made Bakta2, I Made Damriyasa3. INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES, [S.l.], nov. 2012. ISSN 2302-2906. Available at: <https://ojs.unud.ac.id/index.php/ijbs/article/view/3764>. Date accessed: 26 may 2020.
Section
Articles

Keywords

Toxoplasma gondii , protein antigen 30 kDa, ELISA , bioassay.

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