Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS) | Jurnal Veteriner

PDF

Published Mar 2, 2016

Agung Janika Sitasiwi

Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Wayan Tunas Artama Agung Budiyanto Edy Dharmana

Abstract

The aims of this study was to detect the expression of Wingless-type MMTV integration site familymember 4 (Wnt4) protein in the uterus of Swiss Webster mice. Laboratory animals that were used areadult Swiss Webster mice weighing 25-30 grams. Mice were kept and mated in a controlled laboratoryconditions. Pregnancy was determined by the presence of vaginal plug in female mice after breeding.Protein was isolated from the uterus at seven days of gestation. Immunoblotting was performed usingChemiluminescent Western Blot kit. The primary antibody used was anti Wnt-4 antibody with a 1: 1000dilution. The results showed protein bands with molecular weights ranging from 40 kDa showed a positivereaction to the primary antibody that were used so that it can be concluded that the protein is Wnt4protein.

Downloads

Download data is not yet available.

How to Cite

SITASIWI, Agung Janika et al. Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS). Jurnal Veteriner, [S.l.], v. 17, n. 1, p. 71-77, mar. 2016. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/19169>. Date accessed: 05 nov. 2024.

ABNT APA BibTeX CBE EndNote - EndNote format (Macintosh & Windows) MLA ProCite - RIS format (Macintosh & Windows) RefWorks Reference Manager - RIS format (Windows only) Turabian

Keywords

Wnt4; immunoblotting; Swiss Webster

Issue

Vol 17 No 1 (2016)

Section

Articles

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License

Most read articles by the same author(s)

Ida Ayu Pasti Apsari, Wayan Tunas Artama, Sumartono ., I Made Damriyasa, Diagnosis Molekuler Toxoplasma gondii Berdasar Gen Stage Spesifik Takizoit dan Bradizoit pada Ayam Kampung (MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII BASED ON THE TACHYZOITE AND BRADYZOITE STAGE SPECIFIC GENES IN FREE-RANGE CHICKEN) , Jurnal Veteriner: Vol 13 No 1 (2012)
Sorta Basar Ida Simanjuntak, Sukarti Moeljopawiro, Wayan Tunas Artama, Subagus Wahyuono, Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit , Jurnal Veteriner: Vol 12 No 4 (2011)
Ida Ayu Pasti Apsari, Wayan Tunas Artama, Sumartono ., I Made Damriyasa, Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE) , Jurnal Veteriner: Vol 13 No 4 (2012)
I Wayan Suardana, I Nengah Sujaya, Wayan Tunas Artama, Aplikasi Kandidat Pemindai untuk Diagnosis Gen Shiga like toxin-2 dari Escherichia coli O157:H7 (PROBE APLICATION TO DIAGNOSTIC PROGRAME OF SHIGA LIKE TOXIN-2 (STX2) GEN FROM ESCHERICHIA COLI O157:H7) , Jurnal Veteriner: Vol 13 No 4 (2012)
I Wayan Suardana, I Nengah Sujaya, Wayan Tunas Artama, Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES) , Jurnal Veteriner: Vol 14 No 2 (2013)
Enny Yusuf Wachidah Yuniwarti, Widya Asmara, Wayan Tunas Artama, Charles Rangga Tabbu, Virgin Coconut Oil Meningkatkan Aktivitas Fagositosis Makrofag Ayam Pedaging Pascavaksinasi Flu Burung (VIRGIN COCONUT OIL INCREASES THE PHAGOCYTOSIS ACTIVITY OF MACROPHAGE OF BROILER CHICKEN FOLLOWING AVIAN INFLUENZA VACCINATION) , Jurnal Veteriner: Vol 14 No 2 (2013)
Dewa Ayu Agus Sri Laksemi, Wayan Tunas Artama, Mahardika Agus Wijayanti, Seroprevalensi yang Tinggi dan Faktor-Faktor Risiko Toksoplasmosis pada Darah Donor dan Wanita di Bali (HIGH SEROPREVALENCE AND RISK FACTORS OF TOKSOPLASMOSIS AMONG BLOOD DONORS AND WOMEN IN BALI) , Jurnal Veteriner: Vol 14 No 2 (2013)
Rini Widayanti, Trini Susmiati, Wayan Tunas Artama, Keragaman Genetik Gen NADH Dehydrogenase Subunit 6 pada Monyet Hantu (Tarsius Sp.) (GENETIC DIVERSITY STUDY ON NADH DEHYDROGENASE SUBUNIT 6 GENE OF TARSIUS SP.) , Jurnal Veteriner: Vol 14 No 2 (2013)
I Wayan Suardana, Wayan Tunas Artama, Widya Asmara, Budi Setiadi Daryono, Identifikasi Escherichia coli O157:H7 serta Deteksi Gen Shiga Like Toxin 1 dan 2 Asal Feses Hewan, Daging, dan Feses Manusia , Jurnal Veteriner: Vol 11 No 4 (2010)