Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS) | Jurnal Veteriner

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Published Mar 2, 2016

Agung Janika Sitasiwi

Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Wayan Tunas Artama Agung Budiyanto Edy Dharmana

Abstract

The aims of this study was to detect the expression of Wingless-type MMTV integration site familymember 4 (Wnt4) protein in the uterus of Swiss Webster mice. Laboratory animals that were used areadult Swiss Webster mice weighing 25-30 grams. Mice were kept and mated in a controlled laboratoryconditions. Pregnancy was determined by the presence of vaginal plug in female mice after breeding.Protein was isolated from the uterus at seven days of gestation. Immunoblotting was performed usingChemiluminescent Western Blot kit. The primary antibody used was anti Wnt-4 antibody with a 1: 1000dilution. The results showed protein bands with molecular weights ranging from 40 kDa showed a positivereaction to the primary antibody that were used so that it can be concluded that the protein is Wnt4protein.

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How to Cite

SITASIWI, Agung Janika et al. Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS). Jurnal Veteriner, [S.l.], v. 17, n. 1, p. 71-77, mar. 2016. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/19169>. Date accessed: 20 apr. 2024.

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Keywords

Wnt4; immunoblotting; Swiss Webster

Issue

Vol 17 No 1 (2016)

Section

Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License

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