Pengaruh Penambahan Feri Klorida pada Media NPSi terhadap Biomassa dan Kandungan Protein Mikroalga Chaetoceros calcitrans

Abstract

This study aims were to know the effect of ferric chloride (FeCl3) on biomass concentration and
protein content of microalgae Chaetoceros calcitrans and determine the best concentration of ferric
chloride for production of microalgae Chaetoceros calcitrans with highest protein content. This study
was designed with randomized block design with single factor. The factor was different ferric chloride
concentration consist of 4 concentration, i.e. 0 µM, 12 µM, 24 µM and 36 µM. Each treatment was
grouped into 4 based on time of cultivation. The data obtained were analyzed by analyzed of variance
followed by least significant difference test, if a treatment had significant effect. The result showed that
addition of ferric chloride had very significant effect on the b iomass concentration and protein content.
The highest biomass concentration and protein content was obtained from the microalgae Chaetoceros
calcitrans which was cultivated in 36 µM ferric chloride amounted of 4.1 x 10
7
cell/mL and 25.41%.

Keywords: biomass, Chaetoceros calcitrans, Ferric Chloride, NPSi, protein.