AKTIVITAS BIODESULFURISASI DIBENZOTIOFENA DALAM MODEL MINYAK TETRADEKANA PADA RASIO MINYAK AIR DAN KONSENTRASI RESTING SEL ISOLAT BAKTERI SBJ8

Abstract

This study aims to determine the best ratio of oil / water and concentration of resting cells
and determine the time course of resting cells bacteria  isolates SBJ8  to be known the
biodesulfurization capability in two-phase media using media mineral salt sulfur free-tetradecane
(MSSF-TD). Oil/water  ratio used in this study is 1 : 1 , 1 : 2 and 1 : 4 in combination with cell
concentration by OD
660
10, 20,  and 30. The  study was conducted using 200  mg ·  l
–1
dibenzothiophene as a source of sulfur dissolved in tetradecane as a  model of petroleum (oil
phase). Isolates were incubated for 24 hours  at 37°C and the residue analyzed by  gass
cromathography mass selective (GC-MS).  Testing the degradation of resting cells is
accomplished by two replications.  The results showed that the ratio of  oil water 1 : 4 by and cell
concentration OD
660
30 shows the results of the highest degradation of 84.84% and the time
course of 24 hours resting cells show degradation rate dibenzothiophene up to 81.84%.

Keywords :  Biodesulfurization, dibenzothiophene, tetradecane, resting cells