Penentuan Minimum Inhibitory Concentration (MIC) minyak atsiri Acorus calamus terhadap Aspergillus flavus
Abstract
Naskah lontar Bali merupakan dokumentasi budaya Bali masa lampau. Komponen utama daun lontar adalah selulosa. Upaya pencegahan kerusakan naskah lontar dari jamur pendegradasi selulosa seperti Aspergillus flavus sangat diperlukan untuk mempertahankan kearifan lokal Bali. Tujuan dari penelitian ini adalah untuk mengevaluasi nilai Minimum Inhibitory Concentratiton (MIC) dari minyak atsiri daun jangu (Acorus calamus) dalam menghambat pertumbuhan A. flavus secara in vitro. Hasil yang diperoleh yaitu konsentrasi optimum minyak atsiri A. calamus sebesar 5% (v/v), dengan rata-rata diameter zona hambat 11,72 mm (kategori kuat). Nilai MIC dari minyak atsiri daun jangu dalam menghambat A.flavus yaitu 2% v/v dengan rata-rata diameter zona hambat sebesar 7,08 mm. Penelitian ini membuka wawasan baru dalam upaya konservasi naskah lontar Bali akibat dari kerusakan jamur pendegradasi selulosa, dengan memanfaatkan minyak atsiri tumbuhan lokal Bali yaitu daun jangu (A. calamus).
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References
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Tancinova D, Maskova Z, Foltinova D, Stefanikova J, Arvay J. 2018. Effect of essential oils of lamiaceae plants on the Rhizopus spp. Journal of Food Sciences 12(1): 491–498.
Venkatesan R, Karuppiah PS, Arumugam G, Balamuthu K. 2019. β-Asarone exhibits antifungal activity by inhibiting ergosterol biosynthesis in Aspergillus niger ATCC 16888. Proceedings of the National Academy of Sciences India Section B - Biological Sciences 89(1): 173–184.
Yuliatun S, Santoso EM. 2022. Pengaruh konsentrasi natrium hidroksida pada isolasi selulosa dari ampas tebu. Indonesian Sugar Research Journal 2(1): 12–21.
Dinev T, Tzanova M, Velichkova K, Dermendzhieva D, Beev G. 2021. Antifungal and antioxidant potential of methanolic extracts from Acorus calamus, Chlorella vulgaris Beijerinck, Lemna minuta Kunth and Scenedesmus dimorphus (Turpin) Kützing. Applied Sciences (Switzerland) 11(11): 1-13.
Febia A, Mukarlina, Rahmawati. 2020. Aktivitas antifungi ekstrak metanol daun bandotan (Ageratum conyzoides) terhadap Phytophthora sp. (Im5) secara in vitro. Jurnal Protobiont 9(2): 167–174.
Fitria F, Suhartini S, dan Prihandono DS. 2023. Gambaran kapang Aspergillus Sp. pada terasi dalam kemasan tanpa merek di pasar tradisional kota Samarinda. JI-KES (Jurnal Ilmu Kesehatan) 6(2): 147–154.
Hanif A, Zamriyetti Z. 2023. Karakterisasi morfologi cendawan penyebab penyakit busuk pangkal batang pada bawang merah (Allium cepa). AGRIUM: Jurnal Ilmu Pertanian 26(1): 76–82.
Julyeda NKD, Darmayasa, IBG, Hardini J. 2021. Potential of awar-awar leaf methanol extract (Ficus septica Burm. f.) against Aspergillus niger on Balinese lontar. Metamorfosa: Journal of Biological Sciences 8(2): 268-274.
Mohammed GJ, Hameed IH. 2018. Anti-fungal, antitumor and anti-inflammatory activity of Acorus calamus. Indian Journal of Public Health Research and Development 9(3): 254–258.
Nasardin NR, Zainon M, Zulkefle A, Hanafiah MA, Ibrahim M, Rahman AI. 2018. Comparative study on steam distillation and hydro-distillation methods for agarwood oil extraction. International Journal of Applied Engineering Research 13(8): 6253–6256.
Paranatha KAD, Bratayadnya PA, dan Octaviano AL. 2022. Konservasi Naskah lontar dalam fotografi story. Retina Jurnal Fotografi 2(1): 123–132.
Parwanayoni S, Sudirga SK. 2020. Isolasi dan identifikasi senyawa antijamur daun jeringau (Acorus calamus Linn.) sebagai pengendali jamur Athelia rolfsii Sacc. penyebab penyakit busuk batang pada tanaman kedelai. Metamorfosa: Journal of Biological Sciences 7(2):10-12.
Politeo O, Popovic M, Versic Bratincevic M, Koceic P, Nincevic T, Mekinic IG. 2023. Conventional vs. microwave-assisted hydrodistillation: influence on the chemistry of sea fennel essential oil and its by-products. Plants 12(7): 32-43.
Putri D, Nasir N, Agustien A. 2019. Kemampuan jamur akar putih (Rigidoporus microporus (Swartz: Fr.) Van Ov.) dalam mendegradasi limbah selulosa. Metamorfosa: Journal of Biological Sciences 6(1): 102-114.
Rampa E, Manurun S, Sinaga H. 2023. Isolasi fungi endofit dari buah dan daun belimbing wuluh. Jurnal Penelitian Kesehatan Suara Forikes 14(1): 92–96.
Rita WS, Kawuri R, Swantara IMD. 2017. The essential oil contents of jeringau (Acorus calamus L.) rhizomes and their antifungal activity against Candida albicans. Journal of Health Sciences and Medicine 1(1): 33-41.
Rohmah IN, Alif T. 2021. Uji pengembangan spora entomopatogen bunga entomopatogen Lecanicillium lecanii menggunakan haemocytometer. Jurnal Matematika Dan Sains 1(2): 143–150.
Sharma V, Sharma R, Gautam DNS, Kuca K, Nepovimova E, Martins N. 2020. Role of vacha (Acorus calamus Linn.) in neurological and metabolic disorders: Evidence from ethnopharmacology, phytochemistry, pharmacology and clinical study. Journal of Clinical Medicine 9(4): 67-74.
Simanjuntak HA, Butar M. 2019. Uji aktivittas antifungi ekstrak etanol umbi bawang merah (Allium Cepa L.) terhadap Candida albicans dan Pityrosporum Ovale. Eksakta: Jurnal Penelitian dan Pembelajaran MIPA 4(2): 91-98.
Sintawarak P, Uthairatsamee S, Keawgrajang T. 2021. In vitro and in vivo inhibition of Cylindrocladium reteaudii by essential oils of Acorus calamus rhizomes. Environment and Natural Resources Journal 19(1): 34–42.
Szczeblewski P, Wroblewski M, Borzyszkowska-Bukowska J, Bairamova T, Górska J, Laskowski T, Samulewicz, A, Kosno, M., Sobiech, Ł, Polit JT, Kukula-Koch W. 2022. The role of centrifugal partition chromatography in the removal of β-asarone from Acorus calamus essential oil. Scientific Reports 12(1): 1–14.
Tancinova D, Maskova Z, Foltinova D, Stefanikova J, Arvay J. 2018. Effect of essential oils of lamiaceae plants on the Rhizopus spp. Journal of Food Sciences 12(1): 491–498.
Venkatesan R, Karuppiah PS, Arumugam G, Balamuthu K. 2019. β-Asarone exhibits antifungal activity by inhibiting ergosterol biosynthesis in Aspergillus niger ATCC 16888. Proceedings of the National Academy of Sciences India Section B - Biological Sciences 89(1): 173–184.
Yuliatun S, Santoso EM. 2022. Pengaruh konsentrasi natrium hidroksida pada isolasi selulosa dari ampas tebu. Indonesian Sugar Research Journal 2(1): 12–21.
Published
2024-06-29
How to Cite
INDI SWARI, Komang Kartika; DARMAYASA, Ida Bagus Gede; WIBAWA, I Putu Agus Hendra.
Penentuan Minimum Inhibitory Concentration (MIC) minyak atsiri Acorus calamus terhadap Aspergillus flavus.
Jurnal Biologi Udayana, [S.l.], v. 28, n. 1, p. 102-110, june 2024.
ISSN 2599-2856.
Available at: <https://ojs.unud.ac.id/index.php/bio/article/view/114545>. Date accessed: 04 nov. 2024.
doi: https://doi.org/10.24843/JBIOUNUD.2024.v28.i01.p09.
Section
Articles
