Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L.) yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici

Abstract

A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibition
potency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find out
antifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuum
L.) plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4).
Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.
Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili
(C.annuum L.) plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying with
Streptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsici
spore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.
with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82%) against
Fusarium, Streptomyces sp.1 (72%), Streptomyces sp.2 (64%), Streptomyces sp.3 (76%), and
Streptomyces sp. 5 (32%). All Streptomyces suppressed the growth of Fusarium on chili plants in
glass house (p<0,05). Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in control
to 8%.