Streptomyces sp. Sebagai Biofungisida Patogen Fusarium oxysporum (Schlecht.) f.sp. lycopersici (Sacc.) Snyd. et Hans. Penyebab Penyakit Layu Pada Tanaman Tomat (Solanum lycopersicum L.)

Abstract

A research was conducted to isolate Streptomyces sp. of soil Udayana University campus in the
Bukit-Jimbaran, to obtain the most effective Streptomyces sp. which is effective in inhibit the growth of
Fusarium oxysporum f.sp. lycopersici, and to test response of tomato plants with Streptomyces sp.
culture against Fusarium wilt desease. Implementation phases of the research consisted of isolation and
identification of Streptomyces sp, test the inhibition against F. oxysporum f.sp. lycopersici, and in vivo
test used by dyeing the roots of the tomato plant (Solanum lycopersicum) with Fusarium spores and
after 30 seconds the roots were dyeing Streptomyces culture. Furthermore, sterile soil in polybag
watered by Fusarium spores and Streptomyces culture at the same time. Based on morphological
characteristic it found five isolates of Streptomyces sp.. The antagonist test showed Streptomyces sp.
1 had ability (75%) against Fusarium, Streptomyces sp 2 (68,3%), Streptomyces sp. 3 (71,6%),
Streptomyces sp. 4 (63,3%), and Streptomyces sp. 5 (21,6%). All Streptomyces suppressed the
growth of Fusarium on tomato plants in glass house (p<0,05). Streptomyces sp.3 suppressed Fusarium
wilt disease in tomato from 88% in control to 20%.