The effect of dietary seaweed of Caulerpa sp. and Gracilaria sp. on blood glucose levels and histological pancreas of diabetic rats.
Abstract
The aim of this research was to determine the effect of dietary seaweed of Caulerpa sp. and Gracilaria sp. on blood glucose levels and histological pancreas of diabetic rats. A total of 28 male Wistar rats were used in this study. Rats were divided into 4 groups and given different diets i.e. PSN, PSA, PBN and PBS. Bioassay was conducted for 30 days. The analysis conducted on the macronutrient content of the seaweed flour as well as blood glucose levels on day 0, day 1 and day 30 after alloxan injection. At the end of the implementation of bioassay, animal surgery were performed and pancreas organs were taken for histology observation. The results showed that Gracilaria sp. and Caulerpa sp. had a crude fiber content respectively 20.48% and 12.47%. Blood glucose levels in all four groups of mice on day 0 ranged from 123.18 mg / dL - 131.96 mg / dL. Feeding PBN and PBS for 30 days lowered blood glucose levels of mice, respectively for 51.63% (from 384.4 mg / dL to 168.52 mg / dL) and 35.24% (from 328.05 mg / dL be 212.42 mg / dL). Histological observation showed that feeding PBN and PBS capable of helping the regeneration of pancreatic cells, thereby feeding both types of seaweed showed hypoglycemic effects in comparison with standard feed.
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References
AOAC. 1990. Official Methods of Analysis. 15th ed. Vol. 2. Virginia
Astawan, M. Dan Wresdiyati, T. 2004. Diet Sehat Dengan Makanan Berserat. Cetakan I. Tiga Serangkai, Solo.
Dayatri, U.A. 2009. Profil Sel β Pulau Langerhans Jaringan Pankreas Tikus Diabetes Mellitus Yang Diberi Virgin Coconut Oil. Skripsi. Fakultas Kedokteran Hewan, IPB Bogor.
Dewi, R.D., Aulannni’am, Rosdiana, A. 2013. Studi Pemberian Ekstrak Rumput Laut Coklat (Sargassum prismaticum) Terhadap Kadar MDA dan Histologi Jaringan Pankreas Pada Tikus Rattus norvegicus Diabetes Melitus Tipe 1 Hasil Induksi MLD-STZ (Multiple Low Dose-Streptozotocin). Kimia Student Jurnal, 2 (1) 351-357.
Ganong, W.F. 1995. Review of Medical Physiology Lange Medical Publication. San Fransisco, California.
Gomes,K.A. dan Gomes, A.T. 1995. terjemahan E. Sjamsudin dan J.S. Baharsyah. Prosedur Statistik Untuk Penelitian Pertanian. UI Press. Jakarta.
Herpandi, Made, A., Tutik, W., dan Nurheni, S.P. 2006. Perubahan Profil Lipida, Kolesterol Digesta dan Asam Propionat pada Tikus dengan Diet Tepung Rumput Laut. Jurnal teknol. Dan Industri Pangan. XVII No 3, 227 – 232
Julyasih, K.S.M. 2013. Potensi beberapa Jenis Tepung Rumput Laut Untuk Meningkatkan Kadar HDL (High Density Lipoprotein) Plasma Tikus Wistar Hiperkolesterolemia. Rekapangan. 87-91
Kaplan, A. dan Szabo, L. 1983. Clinical Chemistry : Interpretation and Techniques. Lea dan Febiter. Philadelphia.
Ma’aruf, W.F., Ibrahim, R. Nurcahya Dewi, E., Susanto, E. Dan Amalia, U. 2013. Profil Rumput Laut Caulerpa rasemosa dan Gracilaria verrucosa Sebagai Edible Food. Jurnal Saintek Perikanan Vol 9 No 1 : 68-74
Okamoto, H. 1996. Okamoto Model for β-Cell Damage. Recent Advances. Lesson from Animal Diabetes VI. 75th Anniversary of the Insulin Discovery. Ed Eleasar Shafir. Birkhausher, Berlin.
Reeves, P.G., Nielsen, F.H. dan Fahey, G.C. 1993. AIN-93. Purified Diets for Laboratory Rodents : Final Report of the American institute of Nutrition Ad Hoc writing Committee on the Reformulation of AIN-76 Rodent Diet. J. Nutr. 123 : 1939-1953
Shofia, V., Aulannni’am, Mahdi,C.. 2013. Studi Pemberian Ekstrak Rumput Laut Coklat (Sargassum prismaticum) Terhadap Kadar Malondialdehid dan Gambaran Histologi Jaringan Ginjal Pada Tikus Rattus norvegicus Diabetes Melitus Tipe 1. Kimia Student Jurnal, 1 (1) 119-125.
Sudarmadji, S., Haryono, B. dan Suhardi. 1997. Prosedur Analisa Untuk Bahan Makanan dan Pertanian. Yogyakarta: Penerbit Liberty.
