Isolation and Identification of Bacteriocin-Producing Lactic Acid Bacteria from Urutan, Balinese Traditional Fermented sausage

  • Ni Made Sri Dwijastuti Universitas Udayana
  • I Nengah Sujaya Medical Faculty, Udayana University
  • Ni Nengah Dwi Fatmawati Medical Faculty, Udayana University
DOI: https://doi.org/10.24843/metamorfosa.2021.v08.i01.p08

Abstract

This study aimed to isolate and identify lactic acid bacteria (LAB) that could produce bacteriocins from urutan. Urutan is a Balinese sausage that is traditionally produced and naturally fermented. Urutans used in this study were obtained from several traders in the Baturiti, Mengwi, and Tegallalang region, Bali. More than 300 LAB colonies were isolated and underwent inhibition test against the growth of the indicator bacteria. Crude bacteriocins from two isolates, namely J2 and J6, showed inhibition zones against the growth of Lactobacillus plantarum E12.1 and Escherichia coli ATCC 8739. The J6 isolate also inhibited the growth of Enterococcus faecalis ATCC 29212. Both J2 and J6 were gram-positive, bacilli shaped, and showed negative catalase test. Based on the results of the 16S rDNA gene amplification, the two isolates were identified as Lactobacillus plantarum.


Keywords: Lactic Acid Bacteria, Bacteriocin, Lactobacillus plantarum, Urutan

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Author Biographies

I Nengah Sujaya, Medical Faculty, Udayana University

Department of Public Health

Laboratory of Bioscience and Biotechnology, Udayana University

Ni Nengah Dwi Fatmawati, Medical Faculty, Udayana University

Departemen Mikrobiologi Klinik

Published
2021-03-30
How to Cite
DWIJASTUTI, Ni Made Sri; SUJAYA, I Nengah; FATMAWATI, Ni Nengah Dwi. Isolation and Identification of Bacteriocin-Producing Lactic Acid Bacteria from Urutan, Balinese Traditional Fermented sausage. Metamorfosa: Journal of Biological Sciences, [S.l.], v. 8, n. 1, p. 81-88, mar. 2021. ISSN 2655-8122. Available at: <https://ojs.unud.ac.id/index.php/metamorfosa/article/view/67351>. Date accessed: 15 oct. 2025. doi: https://doi.org/10.24843/metamorfosa.2021.v08.i01.p08.
Citation Formats
Issue
Vol 8 No 1 (2021)
Section
Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License