Potensi Ekstrak Metanol Daun Awar-awar (Ficus septica Burm. f.) sebagai Penghambat Aspergillus niger pada Lontar Bali

Abstract

Ficus septica Burm. f. is a wild plant that has antifungal compounds. Exploration of antifungal compounds from natural material important in inhibiting the growth of Aspergillus niger, one of Balinese lontar destructive fungus. Natural material use to reduse lontar damage due to synthetic materials. This study aims to inhibition of awar-awar leaf methanol extract (in vitro). The method used was a complete randomized design with 7 treatments for the concentration of the awar-awar leaf methanolic extract of 10.000, 20.000, 30.000, 40.000, 50.000 ppm (b/v), positive control (nystatin), and negative control (methanol). The minimum inhibitory concentration (MIC) test uses 6 treatments for the concentration of the awar-awar leaf methanolic extract of 100, 500, 1000, 2000 ppm (w/v), positive control (nystatin), and negative control (methanol). Giving the extract to the diffusion well with potato dextrose agar (PDA) media. The research data were analyzed statistically ANOVA (DMRT with 5% significance level) with the SPSS 23 version for windows. The results showed that the awar-awar leaf methanol extract had MIC at a concentration of 500 ppm (w/v). Concentrations of 10.000-50.000 ppm (w/v) indicate the inhibition zone diameter of 20.50 mm, respectively; 23.00 mm; 23.50 mm; 23.75 mm; 24.75 mm. The concentration of 10.000 ppm (w/v) has higher inhibition than the positive control. The concentration of 20.000 ppm (w/v) was the optimum concentration of inhibition of awar-awar leaf methanol extract. Conclusion of this research was awar-awar leaf methanol extract had anti-fungal compounds which inhibited the growth of A. niger. This extract can be used as an alternative to inhibit the growth of fungi that cause lontar damage.

Keyword: Aspergillus niger, destructive fungi, Ficus septica Burm. f.