Elucidation And Inhibition Of Extract Ethanol Sembung Delan (Sphaeranthus Indicus L.) Against Candida Albicans ATCC 1023

Abstract

The sembung delan (Sphaeranthus indicus L.) plant is one of the weeds in Indonesia which has great potential as an alternative antifungal. The purpose of this study was to determine the ability of sembung delan leaf extract to inhibit the fungus Candida albicans ATCC 1023, as a pathogenic species and cause of candidiasis, as well as chemical compounds contained in the extract. The research design used in this study was a completely randomized design (CRD) divided into 7 treatments and 4 repetitions with one treatment factor, namely the concentration of 1% (w/v), 2% (w/v), 3% (w/v), 4% (w/v), 5% (w/v). Positive control (Nystatin) and negative control (Solvent). Phytochemical tests were carried out and analysis of compound content using GC-MS. The results showed that sembung delan leaf extract was able to inhibit C. albicans ATCC 1023 with the greatest inhibition, namely at a concentration of 5% (w/v) with a diameter of 13.00 mm, a minimum concentration of 0.3% (w/v) with a diameter of 5.925 mm. Statistically significantly different (P?0.05) with a concentration of 0.1% (w / v), and 0.2% (w / v) with a diameter of 0.000 mm and an optimum concentration of 4% (w/v) with a diameter of 11.25 mm. Phytochemical test results of the crude extract of sembung delan contain saponins, phenols, steroids, terpenoids, alkaloids, and tannins. For GC-MS analysis, 7 active compounds were obtained with 6 of them known as anti-fungal compounds, namely Thymol; 1-Tetradecanamine, N, N-dimethyl-; Benzene, 2-methoxy-1,2,3-trimethyl-; 6-Octenal, 3,7-dimethyl-; Thymol and Benzyl chloride. The active compounds contained in sembung delan leaf extract have the opportunity to be used as herbal ingredients to control C. albicans ATCC 1023.

Keywords: Minimum Inhibitory Concentration, phytochemicals, thin layer chromatography, gas chromatography mass spectrometry.