IDENTIFIKASI SENYAWA GOLONGAN FENOL DARI EKSTRAK ETANOL DAUN SIRIH HIJAU (PIPER BETLE LINN.) DENGAN METODE KLT-SPEKTROFOTODENSITOMETRI

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Ni Made Pitri Susanti Luh Putu Mirah Kusuma Dewi Harlina Setiawati Manurung I Made Agus Gelgel Wirasuta

Abstract

Phenol compound in Piper betle leaves has several pharmacology activities such as antibacteria, antifungi and antioxidant. The pharmacology activities of a herbal drug are influenced by the phytochemistry content, so in order to do a quality determination that provides phytochemistry profile and consistent pharmacology activities, a standardization is required. Fingerprint is the main standard to perform quality control for herbal drug. TLC-spectrophotodensitometry was used as the method in order to provide fingerprint profile of phenol compound. In this experiment, Piper betle leaves samples were extracted by reflux method using ethanol 96% as the solvent. Identification of phenol compound was done using TLC-spectrophotodensitometry with Silica gel 60 F254 as the stationary phase, toluena: ethyl acetate (93:7 v/v) as the mobile phase, FeCl3 and Folin-Ciocalteau as the reagent. The fingerprint profile of phenol compund was shown in Rf value 0,19; 0,42; and 0,62. Positive results of phenol compound are black spot on FeCl3 colour test and dark blue spot  on Folin-Ciocalteau colour test. Maximum wavelength of phenol compound was 283 nm.

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How to Cite
SUSANTI, Ni Made Pitri et al. IDENTIFIKASI SENYAWA GOLONGAN FENOL DARI EKSTRAK ETANOL DAUN SIRIH HIJAU (PIPER BETLE LINN.) DENGAN METODE KLT-SPEKTROFOTODENSITOMETRI. Metamorfosa: Journal of Biological Sciences, [S.l.], v. 4, n. 1, p. 108-113, mar. 2017. ISSN 2302-5697. Available at: <https://ojs.unud.ac.id/index.php/metamorfosa/article/view/29883>. Date accessed: 12 july 2020. doi: https://doi.org/10.24843/metamorfosa.2017.v04.i01.p16.
Keywords
Piper betle, phenol, fingerprint, TLC-spectrophotodensitometry
Section
Articles