PENGARUH pH DAN LAMA PENYIMPANAN TERHADAP STABILITAS KIMIA STANDAR (+)-KATEKIN

  • Ni Putu Eka Leliqia
  • Yanita Ristanti Purwitadewi
  • I Made Agus Gelgel Wirasuta

Abstract

 (+)-Catechin was a potential compound to be isolated and developed into natural antioxidant product, but it was unstable because it was easy to be oxidized and formed quinone metabolite. This became a problem in its development and isolation process. Factors that can influence the oxidation process of (+)-catechin were pH condition and storage time. Therefore, this research was conducted to know pH and storage time influence towards the (+)-catechin stability. Observation was done in two parameters which was the acid-base pH (pH 1,64-10) and the storage time for 8 days. The influence of both the parameters towards (+)-catechin standard stability was observed by the quinone metabolite formation from (+)-catechin oxidation reaction result. (+)-catechin was declared as unstable if quinone metabolite was formed by absorbance measurement in its maximum wavelength, 409nm with spectrophotometry method. The obtained result showed that (+)-catechin standard was stable in pH 1.64-6 for 8 days storage and was unstable in pH 7-10 showed by the formation of quinone metabolite for 2-8 days storage.

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Published
2015-02-01
How to Cite
LELIQIA, Ni Putu Eka; PURWITADEWI, Yanita Ristanti; WIRASUTA, I Made Agus Gelgel. PENGARUH pH DAN LAMA PENYIMPANAN TERHADAP STABILITAS KIMIA STANDAR (+)-KATEKIN. Indonesian Journal of Legal and Forensic Sciences (IJLFS), [S.l.], v. 5, p. 1-3, feb. 2015. ISSN 2657-0815. Available at: <https://ojs.unud.ac.id/index.php/ijlfs/article/view/37111>. Date accessed: 21 nov. 2024. doi: https://doi.org/10.24843/10.24843/IJLFS.2015.v05.i01.p01.