Bioaktivitas Forbazol-E terhadap Kerusakan Ultrastruktur Dinding Sel Staphylococcus aureus

Abstract

The bioactivity role of phorbazol-E in inhibiting bacterial protein synthesis is unknown. Onthe contrary, phorbazol-A, B, C, and D have been proved to inhibit the bacterial protein synthesis.The effect of forbazol-E on Staphylococcus aureus ATCC25922 cell wall ultrasctucture destructionwas observed. The study used randomized-post test-only control group design which consist of threetreatment (control, treatment I and II) with 9 repeatation. Treatment I: 1 ml overnight culture ofS. aureus was cultured into a mixed of 20 ml Mueller Hinton (MH) broth and 37.5 mg/L phorbazol-E; whereas in treatment II phorbazol-E used was 75.0mg/L. The growth curve of S. aureus wasmonitored using spectrophotometer; whilst the destruction of cell wall ultrastructure was observedusing Transmission Electron Microscope (TEM) at Eijkman Insitute, Jakarta. Phorbazol-E at75.0mg/L significantly caused the destruction of the bacteria cell wall ultrastructure and inhibitedthe bacteria growth in comparison to phorbazol-E at 37.5 mg/L (p<0.05).