EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK ETANOL UMBI UBI JALAR UNGU (Ipomoea batatas L) TERHADAP SEL LINE KANKER PAYUDARA T47D 1

Abstract

Cancer is still one of the leading death causes worldwide. Consuming antioxidants can reduce the cancer incidence.
Anthocyanin that contained in purple sweet potatoes can inhibit DNA damages. In order to prove the activity of purple sweet
potatoes on cancer cell, it is necessary to have some direct research on cancer cell line.
This study is a simple experimental research method. The activities of the anticancer are evaluated from cytotoxic and
antiproliferative effects on cell line breast cancer T47D. Cytotoxic effects is examined with cultured cell stained with tryphan
blue exlusion. Each well are contain of 36,000 cells, which is given ethanol extract purple sweet potatoes with 10 dose variation
starting from 500  g/mL up to 10,000  g/mL replicated three times, then the inhibitory percentage is calculated. Antiproliferative
activity is evaluated by incubating cancer cells that have been given ethanol extract purple sweet potatoes in 3 doses variation;
500, 1,000 and 2,000  g/ml in 24, 48 and 72 hours.
Result of this study showed that ethanol extract purple sweet potatoes had cytotoxic activities on T47D breast cancer
cell line in these dose variations, 500; 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000 and 10,000  g/ml. The
cytotoxic activity on each of dose variations above are 27.56; 42.67; 57.78; 66.67; 72.44; 79.56; 85.33; 87.56; 92.44;100; and
100 %. Ethanol extract purple sweet potatoes showed antiproliferative activities on T47D breast cancer cells in 24, 48, 72 hours
incubation in concentrations 500; 1,000; and 2,000  g/ml; those are 35,700; 29,800; 25,500 cells (24 hours); 72,500; 60,300;
52,600 cells  (48 hours); 149,500;122,600;107,300 cells  (72 hours). In conclusion, ethanol extract purple sweet potatoes had
cytotoxic and antiproliferative activities on cell line breast cancer T47D.