Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura

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Ni Ketut Suwiti Fedik Abdul Rantam I Nengah Kerta Besung

Abstract

A study to detect the presence of bovine major histocompatibility complex (Bo-MHC) in maduracattle has been carried out. Lymphocyte samples were collected from 25 cattle. The protein of lymphocytesamples was analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) andtransferred onto nitrocellulose membrane. Bo-MHC proteins on the membrane were detected by monoclonalantibodies (anti-Bo-MHC class I) and BAQ150A (anti-Bo-MHC class II). The proteins of 48 kDa and 11kDa were detected by MAb B5C which appeared to be the alpha and beta chain of Bo-MHC class I. MAbBAQ150A detected protein band of 26 KDa which was likely to be the beta chain of Bo-MHC class II. Bo-MHC class I dan class II were clearly detected in madura cattle by MAbs which provides a basis for furtherinvestigation on the role of the MHC in the susceptibility the animals to many diseases.

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How to Cite
SUWITI, Ni Ketut; ABDUL RANTAM, Fedik; KERTA BESUNG, I Nengah. Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura. Jurnal Veteriner, [S.l.], v. 9, n. 3, sep. 2008. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/3328>. Date accessed: 01 july 2022.
Keywords
Bovine Major Histocompatibility Complex, madura cattle, monoclonal, antibodies
Section
Articles