THE ESSENTIAL OIL CONTENTS OF JERINGAU (Acorus calamus L.) RHIZOMES AND THEIR ANTIFUNGAL ACTIVITY AGAINST Candida albicans
Abstract
Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus
Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils
from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography-
Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of
rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS
analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool
(1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol
(2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the
growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of
essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.
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References
[1] Astuti Ardiansyah (2005) Leaves Beluntas as Materials of
Antibacterial and Antioxidant. “Daun Beluntas sebagai Bahan
Antibakteri dan Antioksidan”. Available at:
http://www.berita_iptek.com/ cetak_ beritahp? kat=berita &id=33.
[accessed October 27, 2007].
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20
40
60
80
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The inhibition of C.
albicans biomass
formation…
The essential oil concentration (%)
Y= 46,269 + 17,320 ln x
R2= 0,9388
JHSM UNUD Journals, p-ISSN: e-ISSN:
38
[2] Asha, D.S., Ganjewala, D. (2009). Antimicrobial activity of Acorus
calamus (L.) rhizome and leaf extract, Acta Biol. Szeg. 53 (1), pp.
45–49.
[3] Cowan, M.M. (1999) Plant Product as Antimicrobial Agents, Miamy
University, Oxford.
[4] Kokare, C. R. (2007) Pharmaceutical Microbiology Principles and
Applications. Nirali Prakashan.
[5] Pakasi, S.E. dan Salaki, C.L. (2013) Good Crop Cultivation of
Karumenga (Acorus calamus). “Budidaya yang Baik Tanaman
Karumenga (Acorus calamus)”. Available at:
https://seafast.ipb.ac.id/tpc-project/wp-content/.../GAPKarumenga.
pdf. [accessed April 5, 2015].
[6] Phongpaichit, S., Pujenjob, N., Rukachaisirikul, V. and Ongsakul, M.
(2005) Antimicrobial activities of the crude methanol extract of
Acorus calamus Linn. Songklanrimpangin J. Sci. Technol., 27(Suppl.
2), pp. 517-523.
[7] Rita, W.S., Asih, I.A.R.A, Yuliari, N.M. (2016) Potency of Jeringau
rhizomes essential oil (Acorus calamus Linn.) as inhibitors of
Fusarium solani, fungal pathogens causing stem rot diseases on the
dragon fruit. “Potensi minyak atsiri rimpang jeringau (Acorus
calamus Linn.) sebagai penghambat Fusarium solani, jamur patogen
penyebab busuk batang pada buah naga”. Cakra Kimia, 4(2), pp.
120-128.
[8] Setyolaksono, M. P. (2013) Jeringau, Herba With Myriad Benefits.
“Jeringau, Herba Dengan Segudang Manfaat”. BBPPTP Ambon.
Available at: http://ditjenbun.pertanian.go.id/ bbpptpambon/berita-
280-jeringau-herba-dengan-segudang-manfaat.html [accessed April
20, 2015].
[9] Shenvi, S., Vinod, Hegde, R., Kush, A., Reddy, G. C. (2011) A
unique water soluble formulation of β-asarone from sweet flag
(Acorus calamus L.) and its in vitro activity against some fungal
plant pathogens, Journal of Medicinal Plants Research, 5 (20), pp.
5132–5137.
[10] Venskutonis, P.R., and Dagilyte, A. (2003) Composition of Essential
Oil of Sweet Flag (Acorus calamus L.) Leaves at Different Growing
Phases. Journal of Essential Oil Research, 15(5): 313-318. Available
at: http://www.tandfonline.com/doi/abs/10.1080/10412905.2003.
9698598#.VNRt1iw6TIU [accessed April 5, 2015].
