ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta
Abstract
AbstractThis research aims to isolate and phytochemically test of the toxic isolate from ethanol extract of the sponge Hyrtios
erecta taken from the waters of Pari Island beach, Thousand Islands (Jakarta). Extraction of the sponges was carried out by
70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the
stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on
Bhrine Shrimp Lethality Test (BSLT). The compounds of the active isolate were performed by phytochemical test. Based on the
results, it was found that the toxic isolate of Hyrtios erecta sponges has anticancer activity with IC50 of 30,497 ppm. The
anticancer isolate contained alkaloid, steroid, and polyphenol compounds
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References
REFERENCES
[17] Astuti, P., Alam, G., Hartati, M.S., Sari, D., dan
Wahyuono, S. Cytotoxic Test of Alkaloids of Sponge
Petrossian sp: A Potential development as Anticancer.
“Uji Sitotoksik Senyawa Alkaloid dari Spons Petrosia
sp: Potensial Pengembangan sebagai Antikanker”.
Majalah Farmasi Indonesia. 2005. 16 (1) 58-62.
[18] Calabresi, P., and Chabner, B. In: Gilman, G.E., Rall,
T.W., Taylor, O., (Eds). The Pharmacological Basics
of Therapeutis, 8th, ed. Pergamon Press, USA.1991.
[19] Cho, S. G., Valerie, H.L., Wu, X.H. Novel Cytotoxic
Polyprenylated Xanthones from Garcinia gaudichaudii.
Tetrahedron 1998; 54 : 10915-24.
[20] Cram, W.R., Stewart, C.F., In: Herfindal, E.T.,
Gourley, A.B., and Hart, L.L., (Eds). Clinical
Pharmacy and Therapeutics, 5th ed., Maryland USA:
Williams and Wilkins. 1992.
[21] Dira Swantara, I M. dan Rita, W. S. anticancer activity
sponge Hyrtios erecta extract. “Aktivitas antikanker
ekstrak spons Hyrtios erecta”. Indonesian Journal of
Cancer. 2015. 9 (4): 141-145.
[22] Doyle, A, Griffiths, J.B. Cell and Tissue Culture For
Medical Research. New York: John Wiley and Sons
Ltd. 2000.
[23] Green D., Tew, K.D., Hisamatsu, T., and Schein, P.S.
Correlation of Nitrosourea Murine Bone Marrow
Toxicity with Deoxyribonucleic Acid Alkylation and
Chromatin Binding Sites. Biochem. Pharmacol., 1982.
31, 1671-1679.
[24] Herzig R.H., Hines, J.D., and Herzin, G.P. Cerebellar
toxicity with high-dose cytosine arabinoside. J. Clin.
Oncol., 1987. 1: 927-932.
[25] Hoppe, W. F. dan M. J. M. Reichert. Predictable
Annual Mass Release of Gametes By The Coral Reef
Sponge Neofibularia Nolitangere (Porifera:
Demospongiae). Marine biology. 1987. 94: 277-285.
[26] Jemal, A. Murray, T. Ward, E. Samuels, A, Tiwari R.
C., Ghafoor A., Feuer E.J., Thun M.J. Cancer statistics.
CA Cancer J Clin 2005. 55:10-30.
[27] Kumar, P. Praveen, S. Kumaravel and Lalitha, C.
Screening of Antioxidant Activity, Total Phenolics and
IRSC UNUD Journals, p-ISSN: e-ISSN:
20
GC-MS Study of Vitex Negundo. African Journal of
Biochemistry Research. 2010. 4(7): 191-195.
[28] Lorgan, P.C., Crasey, T., and Coleman, R.E. Drugs.
1996. 51 : 571-584.
[29] Maruthupandian, A. and Mohan, V.R. GC-MS
analysis of some bioactive constituents of Pterocarpus
marsupium R. International Journal of ChemTech
Research. 2011. 3(3): 1652-1657.
[30] Meyer, B.N., Ferrigni, N.R., Putman, J.E., Jacobsen,
L.B., Nichols, D.E., dan McLaughin, J.L., Brine
Shrimp: A Convenient General Bioassay for Active
Lant Constituents. Planta Medica, 1982, 45 : 31-34.
[31] Ohashi, K., Winarno, H., Mukao, M., Shibuya, H.
Preparation and Cancer Cell Invasion Inhibitory
Effects of C-16 Alkynic Fatty Acid. Chem. Pharm.
Bull. 2003. 51(4) 463—466.
[32] Pettit, G. R. Natural Product. Natural Product Report,
1996. 59: 812-821.
[33] Sadek, P. The HPLC Solvents Guide. Wiley-
Interscience. 2002.
[34] Steven, Colegate, Russel. Detection, Isolation, and
Structural Determination of Albany. California.
London. Crc Press. 1993.
[35] Sukardiman, Rahman A, Pratiwi, F.N. Pre-screening
test of Anticancer Activity Ether and Methanol Extracts
of Marchantia cf. planiloba Steph. With Death Test
Methods shrimp larvae and densitometry Profile Active
Extracts. “Uji Praskrining Aktivitas Antikanker Ekstrak
Eter dan Ekstrak Metanol Marchantia cf. planiloba
Steph. Dengan Metode Uji Kematian Larva Udang dan
Profil Densitometri Ekstrak Aktif”. Surabaya: Fakultas
Farmasi, Universitas Airlangga. 2004.
[36] Widyastuti, S. Toxicitytest of Iprih Leaf Extract (Ficus
glabella Blume) against Artemia salina Leach and Thin
Layer Chromatography Profile. “Uji Toksisitas Ekstrak
Daun Iprih (Ficus Glabella Blume) Terhadap Artemia
salina Leach Dan Profil Kromatografi Lapis Tipis”.
Skripsi. Surakarta: Fakultas Farmasi, Universitas
Muhammadiyah. 2008.
[37] Winarno, H. Antiproliferative Activity of Octadeca-
8,10,12-trynoic Acid Agains Human Cancer Cell
Lines. Berita-Biologi. 2009. 9(4): 343-348.
[38] Yohana, Arisandi, dan Andriani Y. Efficacy of
Medicinal Plants. “Khasiat Tanaman Obat”. Jakarta:
Pustaka Buku Murah; 2005.
[17] Astuti, P., Alam, G., Hartati, M.S., Sari, D., dan
Wahyuono, S. Cytotoxic Test of Alkaloids of Sponge
Petrossian sp: A Potential development as Anticancer.
“Uji Sitotoksik Senyawa Alkaloid dari Spons Petrosia
sp: Potensial Pengembangan sebagai Antikanker”.
Majalah Farmasi Indonesia. 2005. 16 (1) 58-62.
[18] Calabresi, P., and Chabner, B. In: Gilman, G.E., Rall,
T.W., Taylor, O., (Eds). The Pharmacological Basics
of Therapeutis, 8th, ed. Pergamon Press, USA.1991.
[19] Cho, S. G., Valerie, H.L., Wu, X.H. Novel Cytotoxic
Polyprenylated Xanthones from Garcinia gaudichaudii.
Tetrahedron 1998; 54 : 10915-24.
[20] Cram, W.R., Stewart, C.F., In: Herfindal, E.T.,
Gourley, A.B., and Hart, L.L., (Eds). Clinical
Pharmacy and Therapeutics, 5th ed., Maryland USA:
Williams and Wilkins. 1992.
[21] Dira Swantara, I M. dan Rita, W. S. anticancer activity
sponge Hyrtios erecta extract. “Aktivitas antikanker
ekstrak spons Hyrtios erecta”. Indonesian Journal of
Cancer. 2015. 9 (4): 141-145.
[22] Doyle, A, Griffiths, J.B. Cell and Tissue Culture For
Medical Research. New York: John Wiley and Sons
Ltd. 2000.
[23] Green D., Tew, K.D., Hisamatsu, T., and Schein, P.S.
Correlation of Nitrosourea Murine Bone Marrow
Toxicity with Deoxyribonucleic Acid Alkylation and
Chromatin Binding Sites. Biochem. Pharmacol., 1982.
31, 1671-1679.
[24] Herzig R.H., Hines, J.D., and Herzin, G.P. Cerebellar
toxicity with high-dose cytosine arabinoside. J. Clin.
Oncol., 1987. 1: 927-932.
[25] Hoppe, W. F. dan M. J. M. Reichert. Predictable
Annual Mass Release of Gametes By The Coral Reef
Sponge Neofibularia Nolitangere (Porifera:
Demospongiae). Marine biology. 1987. 94: 277-285.
[26] Jemal, A. Murray, T. Ward, E. Samuels, A, Tiwari R.
C., Ghafoor A., Feuer E.J., Thun M.J. Cancer statistics.
CA Cancer J Clin 2005. 55:10-30.
[27] Kumar, P. Praveen, S. Kumaravel and Lalitha, C.
Screening of Antioxidant Activity, Total Phenolics and
IRSC UNUD Journals, p-ISSN: e-ISSN:
20
GC-MS Study of Vitex Negundo. African Journal of
Biochemistry Research. 2010. 4(7): 191-195.
[28] Lorgan, P.C., Crasey, T., and Coleman, R.E. Drugs.
1996. 51 : 571-584.
[29] Maruthupandian, A. and Mohan, V.R. GC-MS
analysis of some bioactive constituents of Pterocarpus
marsupium R. International Journal of ChemTech
Research. 2011. 3(3): 1652-1657.
[30] Meyer, B.N., Ferrigni, N.R., Putman, J.E., Jacobsen,
L.B., Nichols, D.E., dan McLaughin, J.L., Brine
Shrimp: A Convenient General Bioassay for Active
Lant Constituents. Planta Medica, 1982, 45 : 31-34.
[31] Ohashi, K., Winarno, H., Mukao, M., Shibuya, H.
Preparation and Cancer Cell Invasion Inhibitory
Effects of C-16 Alkynic Fatty Acid. Chem. Pharm.
Bull. 2003. 51(4) 463—466.
[32] Pettit, G. R. Natural Product. Natural Product Report,
1996. 59: 812-821.
[33] Sadek, P. The HPLC Solvents Guide. Wiley-
Interscience. 2002.
[34] Steven, Colegate, Russel. Detection, Isolation, and
Structural Determination of Albany. California.
London. Crc Press. 1993.
[35] Sukardiman, Rahman A, Pratiwi, F.N. Pre-screening
test of Anticancer Activity Ether and Methanol Extracts
of Marchantia cf. planiloba Steph. With Death Test
Methods shrimp larvae and densitometry Profile Active
Extracts. “Uji Praskrining Aktivitas Antikanker Ekstrak
Eter dan Ekstrak Metanol Marchantia cf. planiloba
Steph. Dengan Metode Uji Kematian Larva Udang dan
Profil Densitometri Ekstrak Aktif”. Surabaya: Fakultas
Farmasi, Universitas Airlangga. 2004.
[36] Widyastuti, S. Toxicitytest of Iprih Leaf Extract (Ficus
glabella Blume) against Artemia salina Leach and Thin
Layer Chromatography Profile. “Uji Toksisitas Ekstrak
Daun Iprih (Ficus Glabella Blume) Terhadap Artemia
salina Leach Dan Profil Kromatografi Lapis Tipis”.
Skripsi. Surakarta: Fakultas Farmasi, Universitas
Muhammadiyah. 2008.
[37] Winarno, H. Antiproliferative Activity of Octadeca-
8,10,12-trynoic Acid Agains Human Cancer Cell
Lines. Berita-Biologi. 2009. 9(4): 343-348.
[38] Yohana, Arisandi, dan Andriani Y. Efficacy of
Medicinal Plants. “Khasiat Tanaman Obat”. Jakarta:
Pustaka Buku Murah; 2005.
Published
2017-02-01
How to Cite
SWANTARA, I Made Dira; RITA, Wiwik Susanah; HERNINDY, Rr Anisa.
ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta.
Journal of Health Sciences and Medicine, [S.l.], v. 1, n. 1, p. 16-20, feb. 2017.
ISSN 2622-0555.
Available at: <https://ojs.unud.ac.id/index.php/jhsm/article/view/34808>. Date accessed: 04 nov. 2024.
doi: https://doi.org/10.24843/JHSM.2017.v01.i01.p05.
Section
Articles
