ISOLATION AND IDENTIFICATION OF BACTERIA FROM GOAT MILK PERANAKAN ETAWA INDICATED CLINICAL MASTITIS IN SEVERAL S
Abstract
The purpose of this study was to determine the types of bacteria as a cause of clinical mastitis in goats Peranakan etawa in Banyuwangi. In this study, the sample used goat milk peranakan etawa in District Pesanggaran, Rogojampi, Songgon and Srono in Banyuwangi. As many as 11 samples were taken then analyzed in Laboratory Microbiology, Faculty of Veterinary Medicine Udayana University. Phase isolation and identification of samples were done to identify the bacteria that cause clinical mastitis through several stages of growth on Sheep Blood Agar (SBA), Gram stain, catalase test, test oxidase, growth in the TSIA and continued test Indol, Methyl Red, Voges Proskauer and citrate (IMViC ). Based on the results, namely mastitis-causing bacteria; Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp and Listeria sp. The conclusion of this study disease mastitis in goats Peranakan etawa in the District of Bayuwangi caused by Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp and Listeria sp.
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References
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Carter GR, Darla JW. 2004. Essentials of Veterinary Bacteriology and Mycology. Sixth Edition. Lowa States Press. Lowa. USA.
Ceballos LS, Morales ER, Adarve GDLT, Castro JD, Martinez LP, Sampelayo MRS. 2009. Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology. J Food Comp Anal 22: 322-329.
Contreras A, Corrales JC, Sanches A, Sierra D. 1997. Persistence of subclinical intramammary pathogen in goats throughout lactation. J Dairy Sci 80: 2815-2819.
Contreras A, Luengo C, Sanchez A, Corrales JC. 2003. The role of intramamary pathogens in dairy goats. Livest Prod Sci 79: 273-283.
Dewi AK. 2013. Isolasi, identifikasi dan uji sensitivitas Staphylococcus aureus terhadap amoxicillin dari sampel susu kambing peranakan ettawa (PE) penderita matitis di wilayah Girimulyo, Kulonprogo, Yogyakarta. J Sain Vet 31(2): 138-150.
Ebrahimi A, Lotfalian SH, Karimi S. 2007. Drug resistance in isolated bacteria from milk of sheep and goats with subclinical mastitis in Shahrekord District. Iranian J Vet Res 8(1): 76-79.
Ebrahimi A, Naser S, Somayeh S, Pezman M. 2010. Characteristics of Staphylococci isolated from mastitic goat milk in Iranian dairy herds. J Vet World 3(5): 205-208.
Hawari AD, Maher O, Saddam Sh, Awaisheh, Hala I, Al-Daghistani, Amal AA, Sharaf SO, Issam MQ, Hanee MA, Jafar EQ. 2014. Prevalence of mastitis pathogens and their resistance againts antimicrobial agents in awassi sheep in Al-BAlqa Province of Jordan. Am J Anim Vet Sci 9(2): 116-121.
Hemraj V, Diksha, Avneet. 2013. A review on commonly used biochemichal test for bacteria. J Life Sci 1(10): 1-7.
Islam MA, Samad MA, Rahman AKM. 2011. Bacterial pathogens and risk factors associated with mastitis in black bengal goats IN Bangladesh. Bangl J Vet Med 9(2): 155-159.
Isnel NB, Sukru K. 2012. Isolation of microorganism from goats with subclinical mastitis and detection of antibiotics susceptibility. J Anim Health Prod Hyg 1(2): 106-112.
Leitner G, Merin U, Silanikove N. 2014. Changes in milk composition as affected by subclinical mastitis in goats. J Dairy Sci 87: 1719-1726.
Leitner G, Silanikove N, Merin U. 2008. Estimate of milk and curd yield loss of sheep and goats with intramammary infection and its relation to somatic cell count. J Small Rumin Res 74: 221-225.
Lumantouw, SF, Febby EFK, Sendy BR, Marina FOS. 2013. Isolasi dan identifikasi bakteri yang toleran terhadap fungisida mankozeb pada lahan pertanian tomat di Desa Tempok, Kecamatan Tompaso, Sulawesi Utara. Fakultas MIPA Universitas Sam Ratulangi. Manado.
Marimuthu M Faez FJA. 2014. Prevalence and antimicrobial resistance assessment of subclinical mastitis in milk samples from selected farms. Am J Anim Vet Sci 9(1): 65-70.
Najeeb MF, Anjum AA, Ahmad MUD, Khan HM, Ali MA, Sattar MMK. 2013. Bacterial etiology of subclinical mastitis in dairy goats and multiple drug resistance of the isolates. J Anim Plant Sci 23(6): 1541-1544.
Nofu K, Siti K, Irwan L. 2014. Isolasi dan karakteristik bakteri pendegradasi selulosa pada ampas tebu kuning (Bagasse). J Protobionat 3(1): 25-33.
Purnomo A, Hartatik K, Siti IOS, Soegiyono. 2006. Isolasi dan karakterisasi Staphylococcus aureus asal susu kambing peranakan etawa. Media Ked Hewan 22(3): 142-147.
Putra RR, Masdiana CP, Dyah KW. 2012. Pengaruh penggunaan biosurfaktan asal pseudomonas sp dengan media tumbuh air rendaman kedelai terhadap kadar Total Suspended Solid (TSS) dan lemak pada bioremediasi limbah cair rumah potong ayam (RPA). Pendidikan Sarjana Kedokteran Hewan. Malang.
Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC. 2002. Veterinary Microbiology and Microbial Disease. Blackwell Science, inc. USA
Sharif A, Muhammad U, Ghulam M. 2009. Mastitis control in dairy production. J.Agric Soc Sci 5: 102-105.
Shearer JK, Harris Jr B. 2003. Mastitis in Dairy Goats. University of Florida
Singh P, Alka P. 2008. Isolation of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes from milk products sold under market conditions at Agra Region. J Acta Agric Slovenica 1(1): 83-88.
Suwito W, Wahyuni AETH, Widagdo SN, Bambang S. 2013. Isolasi dan identifikasi bakteria mastitis klinis pada kambing peranakan etawah. J Sain Vet 31: 49-54.
Syukur A, Bambang S. 2014. Bisnis Pembibitan Kambing. Penebar Swadaya. Jakarta.
Published
2017-02-12
How to Cite
ISNAN, M Hasan; GELGEL, Ketut Tono Pasek; SUARJANA, I Gusti Ketut.
ISOLATION AND IDENTIFICATION OF BACTERIA FROM GOAT MILK PERANAKAN ETAWA INDICATED CLINICAL MASTITIS IN SEVERAL S.
Buletin Veteriner Udayana, [S.l.], feb. 2017.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/28736>. Date accessed: 22 nov. 2024.
Section
Articles
Keywords
Mastitis klinis; isolasi; bakteri
