ENTEROBACTER SP. IN BALI CATTLE ACCORDING TO GEOGRAPHIC AND LEVEL OF MATURITY AND ITS SENSITIVITY PATTERNS TO ANTIBIOTICS
Abstract
An observational study in order to determine the distribution Enterobacter spin Bali cattle at different geographical area (highland and lowland) and according to the animal maturity (calves, heifers, adult) have been undertaken. Isolation and identification of bacteria based on the method of Carter and Cole with slightly modification and the antibiotic sensitivity test were determined using the disc diffusion method by Kirby and Bauer. The Enterobacter sp. was isolated from 16 (13.3%) out of the 120 nasal swab samples,where 8 (6.7%), 6(5.0%), and 2 (1.7%) was isolated from calf, heifers, and adults, respectively. Meanwhile, 6 (5.0%) Enterobacter sp. was isolated from the highland and the remains (8.3%) from the lowland. Antibiograms showed that 13 of the 16 isolates (81.2%) weresensitive to sulfamethoxazole and the remains (18.8%) intermediate. While 12 (75.0%), 1 (6.2%), and 3 (18.8%) isolates weresensitive, intermediate, and resistant to ampicillin, respectively. To oxytetracycline 8 (50.0%) isolates were sensitive, 4 (25.0%) isolates were intermediate, and 4 (25.0%) isolates were resistant. Generally, the Enterobacter sp isolates were sensitive to all the antibiotics tested.
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References
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Asaye M, Biyazen H, Bezie M. 2015. Isolation and characterization of respiratory tract bacterial species from domestic animals with pneumonic lungs from elphora abattoir, ethiopia. International J. Microbiol. Res. 6(1): 13-19.
Badan Pusat Statistik Provinsi Bali. 2014. Geografi dan Iklim. http://www.bali.bps.go.id. (Diakses Pada 5 Januari 2016).
Bhaskara IBM, Budiasa IK, Tono K. 2012. Uji kepekaan Escherichia coli sebagai penyebab kolibasilosis pada babi muda terhadap antibiotika oksitetrasiklin, streptomisin, kanamisin dan gentamisin. Bul. Vet. Udayana. 1(2): 186-201.
Bisping W, Amtsberg G. 1988. Colour atlas for the diagnosis of bacterial pathogens in animals. Paul Parey Scientific Publishares. Berlin And Hamburg.
Brown AN, Smith K, Samuels TA, Jiangrui L, Obare SO, Scotta ME. 2012. Nanoparticles functionalized with ampicillin destroy multiple-antibiotic-resistant isolates of Pseudomonas aeruginosa and Enterobacter aerogenes and methicillin-resistant Staphylococcus aureus. Appl. Environ. Microbiol. 78(8): 2768-2774.
Carter GR, ColeJr JR. 1990. Diagnostic procedures in veterinary bacteriology and mycology. 5th Ed. Academic Press, Inc Harcourt Brace Jovanovich, Publishers.
Gabinaitiene A, Siugzdaite J, Zilinskas H, Siugzda R, Petkevicius S. 2011. Mycoplasma bovisand bacterial pathogens in the bovine respiratory tract. Vet. Medicina. 56(1): 28–34.
Handriana IKJ, Suarjana GK, Tono K. 2015. Pola kepekaan Escherichia coli yang diisolasi dari feses burung kicau penderita diare terhadap antibiotik sulfametoksazol, ampisilin, dan oksitetrasiklin. Bul. Vet. Udayana. 7(2): 157-160.
Hartel H, Nikunen S, Neuvonen E, Tanskanen R, Kivela S, Aho P, Soveri T, Saloniem H. 2004. Viraland bacterial pathogens in bovine respiratory disease in Finland. Actaveterinaria Scandinavica, 45: 193-200.
Kardiasih S. 2004. Performans sapi bali berdasarkan ketinggian tempat di daerah transmigrasi Bengkulu: Performans Pertumbuhan. J. Ilmu-Ilmu Pertanian Indonesi. 6(1): 50-56.
Koneman, E.W., Allen, S.D., Dowell, V.R. dan Sommers, H.M. 1983. Color atlas and Textbook of Diagnostic Microbiology. 2nd Ed, J.B. Lippincott Company. Philadelphia.
Levy SB, Marshall B. 2004. Antibacterial resistance worldwide: causes, challenges and responses. Nat. Med. Supp. 10(12): 122-129.
Mader TL, Davis MS, Brown-Brandl TM. 2006. Environmental factors influencing heat stressin feedlot cattle. J. Anim. Sci. 84: 712-719.
Ojo OE, Ogunyinka OG, Agbaje M, Okubuye JO, Kehinde OO, Oyekunle MA. 2012. Enterobacteriaceae isolated from free-range chickens in Abeokuta, Nigeria. Veterinarski. Arhiv. 82(6): 577-589.
Publi Health England (PHE). 2015. Identification of Enterobacteriaceae. UK Standards For Microbiology Investigations (Smis).
Sari SRPW, Suartha IN, Batan IW. 2016. Status praesen pedet sapi bali. Bul. Vet. Udayana. 8(1): 36-43.
Sartono IM, Treman IW, Suditha IN. 2013. Pemetaan persebaran lahan perkebunan sistem tumpang sari beda umur di Kecamatan Kintamani Kabupaten Bangli. Jurusan Pendidikan Geografi Undiksha, Jalan Udayana Kampus Temgah Undiksha.
Singh AK, Rathore G, Singh V, Mani I, Mishra SK, Mishra BN, Verma OP. 2009. Bacterial resistance to oxytetracycline in different life stages of Indian freshwater carp aquaculture system. Int. J. Microbiol. Res. 1(1): 25-34.
Suharsa IWA, Suarjana IGK, Tono K. 2015. Pola kepekaan Escherichia coli yang diisolasi dari feses broiler penderita diare terhadap sulfametoksazol, ampisilin dan oksitetrasiklin. Bul. Vet. Udayana. 7(2): 101-106.
Suwiti NK. 2008. Identifikasi daging sapi bali dengan metode histologi. Majalah Ilmiah Peternakan. 11(1): 0853-8999.
Weese JS. 2008. Investigation of Enterobacter cloacae infections at a small animal veterinary teaching hospital, Vet. Microbiol. 130(3-4): 426-428.
Wilberger MS, Anthony KE, Sasha R, McClain M, Luiz EB. 2012. Beta-lactam antibiotic resistance among Enterobacter spp. Isolated from Infection in Animals. Adv. Microbiol. 2: 129-137.
Yenny, Herwana E. 2007. Resistensi dari bakteri enterik: aspek global terhadap antimikroba. Universa Medicina. 26(1): 46-56.
Published
2018-08-27
How to Cite
SUARNATA, I Wayan; SUARJANA, I Gusti Ketut; TENDEN ROMPIS, Aida Louise.
ENTEROBACTER SP. IN BALI CATTLE ACCORDING TO GEOGRAPHIC AND LEVEL OF MATURITY AND ITS SENSITIVITY PATTERNS TO ANTIBIOTICS.
Buletin Veteriner Udayana, [S.l.], p. 154-161, aug. 2018.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/32456>. Date accessed: 19 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2018.v10.i02.p08.
Section
Articles
