Isolasi dan Identifikasi Bakteri dari Susu Kambing Peranakan Etawa Terindikasi Mastitis Klinis di Beberapa Kecamatan di Kabupaten Banyuwangi
Abstrak
Tujuan dari penelitian ini adalah untuk mengetahui jenis-jenis bakteri sebagai penyebab mastitis klinis pada kambing peranakan etawa di Kabupaten Banyuwangi. Pada penelitian ini sampel yang digunakan adalah susu kambing peranakan etawa di Kecamatan Pesanggaran, Rogojampi, Songgon dan Srono di Kabupaten Banyuwangi. Sampel yang diambil sebanyak 11 sampel, kemudian dianalisis laboratorik di laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Udayana. Tahap isolasi dan identifikasi sampel sampai teridentifikasinya bakteri penyebab mastitis klinis melalui beberapa tahap yaitu penumbuhan pada Sheep Blood Agar (SBA), pewarnaan Gram, uji katalase, uji oksidase, penumbuhan pada TSIA serta dilanjutkan uji Indol, Methyl Red, Voges Proskauer dan Citrat (IMVIC). Berdasarkan hasil penelitian didapatkan bakteri penyebab mastitis yaitu; Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp, dan Listeria sp. Kesimpulan dari penelitian ini penyakit mastitis pada kambing Peranakan etawa di Kabupaten Bayuwangi disebabkan oleh Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp, dan Listeria sp.
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Referensi
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Ebrahimi A, Naser S, Somayeh S, Pezman M. 2010. Characteristics of Staphylococci isolated from mastitic goat milk in Iranian dairy herds. J Vet World 3(5): 205-208.
Hawari AD, Maher O, Saddam Sh, Awaisheh, Hala I, Al-Daghistani, Amal AA, Sharaf SO, Issam MQ, Hanee MA, Jafar EQ. 2014. Prevalence of mastitis pathogens and their resistance againts antimicrobial agents in awassi sheep in Al-BAlqa Province of Jordan. Am J Anim Vet Sci 9(2): 116-121.
Hemraj V, Diksha, Avneet. 2013. A review on commonly used biochemichal test for bacteria. J Life Sci 1(10): 1-7.
Islam MA, Samad MA, Rahman AKM. 2011. Bacterial pathogens and risk factors associated with mastitis in black bengal goats IN Bangladesh. Bangl J Vet Med 9(2): 155-159.
Isnel NB, Sukru K. 2012. Isolation of microorganism from goats with subclinical mastitis and detection of antibiotics susceptibility. J Anim Health Prod Hyg 1(2): 106-112.
Leitner G, Merin U, Silanikove N. 2014. Changes in milk composition as affected by subclinical mastitis in goats. J Dairy Sci 87: 1719-1726.
Leitner G, Silanikove N, Merin U. 2008. Estimate of milk and curd yield loss of sheep and goats with intramammary infection and its relation to somatic cell count. J Small Rumin Res 74: 221-225.
Lumantouw, SF, Febby EFK, Sendy BR, Marina FOS. 2013. Isolasi dan identifikasi bakteri yang toleran terhadap fungisida mankozeb pada lahan pertanian tomat di Desa Tempok, Kecamatan Tompaso, Sulawesi Utara. Fakultas MIPA Universitas Sam Ratulangi. Manado.
Marimuthu M Faez FJA. 2014. Prevalence and antimicrobial resistance assessment of subclinical mastitis in milk samples from selected farms. Am J Anim Vet Sci 9(1): 65-70.
Najeeb MF, Anjum AA, Ahmad MUD, Khan HM, Ali MA, Sattar MMK. 2013. Bacterial etiology of subclinical mastitis in dairy goats and multiple drug resistance of the isolates. J Anim Plant Sci 23(6): 1541-1544.
Nofu K, Siti K, Irwan L. 2014. Isolasi dan karakteristik bakteri pendegradasi selulosa pada ampas tebu kuning (Bagasse). J Protobionat 3(1): 25-33.
Purnomo A, Hartatik K, Siti IOS, Soegiyono. 2006. Isolasi dan karakterisasi Staphylococcus aureus asal susu kambing peranakan etawa. Media Ked Hewan 22(3): 142-147.
Putra RR, Masdiana CP, Dyah KW. 2012. Pengaruh penggunaan biosurfaktan asal pseudomonas sp dengan media tumbuh air rendaman kedelai terhadap kadar Total Suspended Solid (TSS) dan lemak pada bioremediasi limbah cair rumah potong ayam (RPA). Pendidikan Sarjana Kedokteran Hewan. Malang.
Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC. 2002. Veterinary Microbiology and Microbial Disease. Blackwell Science, inc. USA
Sharif A, Muhammad U, Ghulam M. 2009. Mastitis control in dairy production. J.Agric Soc Sci 5: 102-105.
Shearer JK, Harris Jr B. 2003. Mastitis in Dairy Goats. University of Florida
Singh P, Alka P. 2008. Isolation of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes from milk products sold under market conditions at Agra Region. J Acta Agric Slovenica 1(1): 83-88.
Suwito W, Wahyuni AETH, Widagdo SN, Bambang S. 2013. Isolasi dan identifikasi bakteria mastitis klinis pada kambing peranakan etawah. J Sain Vet 31: 49-54.
Syukur A, Bambang S. 2014. Bisnis Pembibitan Kambing. Penebar Swadaya. Jakarta.
Carter GR, Darla JW. 2004. Essentials of Veterinary Bacteriology and Mycology. Sixth Edition. Lowa States Press. Lowa. USA.
Ceballos LS, Morales ER, Adarve GDLT, Castro JD, Martinez LP, Sampelayo MRS. 2009. Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology. J Food Comp Anal 22: 322-329.
Contreras A, Corrales JC, Sanches A, Sierra D. 1997. Persistence of subclinical intramammary pathogen in goats throughout lactation. J Dairy Sci 80: 2815-2819.
Contreras A, Luengo C, Sanchez A, Corrales JC. 2003. The role of intramamary pathogens in dairy goats. Livest Prod Sci 79: 273-283.
Dewi AK. 2013. Isolasi, identifikasi dan uji sensitivitas Staphylococcus aureus terhadap amoxicillin dari sampel susu kambing peranakan ettawa (PE) penderita matitis di wilayah Girimulyo, Kulonprogo, Yogyakarta. J Sain Vet 31(2): 138-150.
Ebrahimi A, Lotfalian SH, Karimi S. 2007. Drug resistance in isolated bacteria from milk of sheep and goats with subclinical mastitis in Shahrekord District. Iranian J Vet Res 8(1): 76-79.
Ebrahimi A, Naser S, Somayeh S, Pezman M. 2010. Characteristics of Staphylococci isolated from mastitic goat milk in Iranian dairy herds. J Vet World 3(5): 205-208.
Hawari AD, Maher O, Saddam Sh, Awaisheh, Hala I, Al-Daghistani, Amal AA, Sharaf SO, Issam MQ, Hanee MA, Jafar EQ. 2014. Prevalence of mastitis pathogens and their resistance againts antimicrobial agents in awassi sheep in Al-BAlqa Province of Jordan. Am J Anim Vet Sci 9(2): 116-121.
Hemraj V, Diksha, Avneet. 2013. A review on commonly used biochemichal test for bacteria. J Life Sci 1(10): 1-7.
Islam MA, Samad MA, Rahman AKM. 2011. Bacterial pathogens and risk factors associated with mastitis in black bengal goats IN Bangladesh. Bangl J Vet Med 9(2): 155-159.
Isnel NB, Sukru K. 2012. Isolation of microorganism from goats with subclinical mastitis and detection of antibiotics susceptibility. J Anim Health Prod Hyg 1(2): 106-112.
Leitner G, Merin U, Silanikove N. 2014. Changes in milk composition as affected by subclinical mastitis in goats. J Dairy Sci 87: 1719-1726.
Leitner G, Silanikove N, Merin U. 2008. Estimate of milk and curd yield loss of sheep and goats with intramammary infection and its relation to somatic cell count. J Small Rumin Res 74: 221-225.
Lumantouw, SF, Febby EFK, Sendy BR, Marina FOS. 2013. Isolasi dan identifikasi bakteri yang toleran terhadap fungisida mankozeb pada lahan pertanian tomat di Desa Tempok, Kecamatan Tompaso, Sulawesi Utara. Fakultas MIPA Universitas Sam Ratulangi. Manado.
Marimuthu M Faez FJA. 2014. Prevalence and antimicrobial resistance assessment of subclinical mastitis in milk samples from selected farms. Am J Anim Vet Sci 9(1): 65-70.
Najeeb MF, Anjum AA, Ahmad MUD, Khan HM, Ali MA, Sattar MMK. 2013. Bacterial etiology of subclinical mastitis in dairy goats and multiple drug resistance of the isolates. J Anim Plant Sci 23(6): 1541-1544.
Nofu K, Siti K, Irwan L. 2014. Isolasi dan karakteristik bakteri pendegradasi selulosa pada ampas tebu kuning (Bagasse). J Protobionat 3(1): 25-33.
Purnomo A, Hartatik K, Siti IOS, Soegiyono. 2006. Isolasi dan karakterisasi Staphylococcus aureus asal susu kambing peranakan etawa. Media Ked Hewan 22(3): 142-147.
Putra RR, Masdiana CP, Dyah KW. 2012. Pengaruh penggunaan biosurfaktan asal pseudomonas sp dengan media tumbuh air rendaman kedelai terhadap kadar Total Suspended Solid (TSS) dan lemak pada bioremediasi limbah cair rumah potong ayam (RPA). Pendidikan Sarjana Kedokteran Hewan. Malang.
Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC. 2002. Veterinary Microbiology and Microbial Disease. Blackwell Science, inc. USA
Sharif A, Muhammad U, Ghulam M. 2009. Mastitis control in dairy production. J.Agric Soc Sci 5: 102-105.
Shearer JK, Harris Jr B. 2003. Mastitis in Dairy Goats. University of Florida
Singh P, Alka P. 2008. Isolation of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes from milk products sold under market conditions at Agra Region. J Acta Agric Slovenica 1(1): 83-88.
Suwito W, Wahyuni AETH, Widagdo SN, Bambang S. 2013. Isolasi dan identifikasi bakteria mastitis klinis pada kambing peranakan etawah. J Sain Vet 31: 49-54.
Syukur A, Bambang S. 2014. Bisnis Pembibitan Kambing. Penebar Swadaya. Jakarta.
Diterbitkan
2017-02-12
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ISNAN, M Hasan; GELGEL, Ketut Tono Pasek; SUARJANA, I Gusti Ketut.
Isolasi dan Identifikasi Bakteri dari Susu Kambing Peranakan Etawa Terindikasi Mastitis Klinis di Beberapa Kecamatan di Kabupaten Banyuwangi.
Buletin Veteriner Udayana, [S.l.], feb. 2017.
ISSN 2477-2712.
Tersedia pada: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/28736>. Tanggal Akses: 06 july 2026
Terbitan
Bagian
Articles
Kata Kunci
Mastitis klinis; isolasi; bakteri
