Pengaruh Metode Sterilisasi Permukaan terhadap Kultur Biji Jeruk Japansche Citroen (JC) Citrus limonia Osbeck
Abstract
Effect of Surface Sterilization Methods on Seed Culture of JC Citrus limonia Osbeck Japansche Citroen (JC) Citrus limonia Osbeck is one type of citrus that is commonly used as rootstock. JC seed propagation through in vitro culture will be more effective. The initiation stage is the first step of in vitro culture to obtain microbial-free explants and initiate the initial growth. At this stage the problem of contamination becomes the main limiting factor. This study aims to obtain the appropriate method of sterilizing of JC orange seed explants in in vitro culture propagation. A total of four sterilization methods have been tested at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. The sterilization material used is tween 80, fungicide, 20-30% NaOCl and 90% alcohol. The variables observed in this study were the time of appearance of contamination, the percentage of types of contaminants (bacteria and fungi), the percentage of sterile explants and the percentage of live explants after 28 days of culture. The results showed that the fourth method was the optimal sterilization method with the lowest contamination level of 30% of the other three methods with the highest explant survival rate of 60% compared to the other three methods.
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References
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Adenaike, O., and Abakpa, G. O. (2021). Antioxidant Compounds and Health Benefits of Citrus Fruits. European Journal of Nutrition & Food Safety, 13(2): 65–74.
Adji, D., Zuliyanti dan Larashanty, H. (2007). Perbandingan Efektivitas Sterilisasi Alkohol 70%, Inframerah, Otoklaf dan Ozon Terhadap Pertumbuhan Bakteri Bacillus subtilis. J. Sain Vet, 25(1): 17-24.
Akhtar, N., Rehman, M.U., Khan, H.M.S., Rasool, F., Saeed, T., and Murtaza, G. (2011). Penetration Enhancing Effect of Polysorbate 20 and 80 on the In Vitro Percutaneous Absorption of L-Ascorbic Acid. Tropical Journal of Pharmaceutical Research, 10(3): 281-288.
Almeida, L. S., Sousa, E. M. R., da Silva, P. H., Oliveira, T. M., de Almeida, A. A. F., Filho, M. A. C., Soares Filho, W. D. S., and Gesteira, A. da S. (2019). The Morphological Study and Gene Expression Analysis in Citrus Hybrid with a Short Juvenile Period. Crop Breeding and Applied Biotechnology. 19(3): 262-268.
Andrini, A., Suharsi, T. K., dan Surahman, M. (2013). Studi Poliembrioni dan Penentuan Tingkat Kemasakan Fisiologis Benih Japansche Citroen (JC) Berdasarkan Warna Kulit Buah. Jurnal Hortikultura, 23(3): 195-202.
Aseesh, P., and Sushma, T. (2016). Efficient Micropropogation of Citrus sinensis (L.) Osbeck from Cotyledonary Explants Suitable for the Development of Commercial Variety. African Journal of Biotechnology, 15(34): 1806–1812.
Ayu, Y. P. K., Arry, S., Mudji, S., dan Lilik, S. (2017). Studi Poliembrioni pada Benih Batang Bawah Jeruk Japansche Citroen (JC). Jurnal Produksi Tanaman, 5(9): 1497-1504.
Berutu, F. S., Dita, A., dan Darmawan, S., 2018. Pertumbuhan Tunas Citrumelo (Citrus paradise Macfaden cv. Duncan x Poncirus trifoliate (L.) pada Berbagai Konsentrasi Nutrisi untuk Pertumbuhan Lambat (Slow Growth) secara In Vitro. Jurnal Produksi Tanaman, 6(1): 83-91.
Eed, A. M., Begum, H., Sivaramakrishnan, S., Silva, J. A. T. da, Amrender-Reddy, S., and Al-gabal, A. Q. (2011). Global Science Books Rapid Protocol for in Vitro Multiplication of Citrus limonia Osbeck Rootstock. International Journal of Plant Developmental Biology, 5(1): 78–82.
Fitriani, Y., Wijana, G., dan Darmawati, I. A. P. (2019). Teknik Sterilisasi dan Efektivitas 2,4-D Terhadap Pembentukan Kalus Eksplan Daun Nilam (Pogostemon cablin Benth.) In Vitro. J. Agric. Sci. and Biotechnol, 8(1): 41-52.
Gerolino, E. F., Talita, P. C. C., Arquimedes, S. F., Eliezer, R. S., Regina, A. C. G., and Arildo, J. B. D. O. (2015). Evaluation of Limonoid Production in Suspension Cell Cultur of Citrus sinensis. Revista Brasileira de Farmacognosia. 25: 455-461.
Hanif, Z. (2020). Pengembangan Agribisnis Jeruk Nusantara. Balai Penelitian Tanaman Jeruk dan Buah Subtropika.
Hashim, S. N., Ghazali, S. Z., Sidik, N. J., Chia-Chay, T., and Saleh, A. (2021). Surface Sterilization Method for Reducing Contamination of Clinacanthus nutans Nodal Explants Intended for In-vitro Culture. E3S Web of Conferences, 306: 1-8.
Jayanti, M. A. D., Sugiyatnto, A., Roviq, M., dan Magfhoer, D. (2015). Kompabilitas Tujuh Varietas Calon Interstock Tanaman Jeruk pada Batang Bawah Japansche Citroen (JC). Jurnal Produksi Tanaman, 10(10): 1-10.
Jenks, M. A., Hasegawa, P. M., and Jain, S. M. (2007). Advances in Nolecular Breeding Toward Drought and Salt Tolerant Crops. Springer.
Khan, U. M., Sameen, A., Aadil, R. M., Shahid, M., Sezen, S., Zarrabi, A., Ozdemir, B., Sevindik, M., Kaplan, D. N., Selamoglu, Z., Ydyrys, A., and Anitha, T. (2021). Citrus Genus and Its Waste Utilization: A Review on Health-Promoting Activities and Industrial Application. Review Article, 1: 1-17.
Mahmoud, S. N., and Al-Ani, N. K. (2016). Effect of Different Sterilization Methods on Contamination and Viability of Nodal Segments of Cestrum nocturnum L. International Journal of Research Studies in Biosciences (IJRSB), 4(1): 4-9.
Meghwal, P. R., Sharma, H. C., and Singh, S. K. (2000). Effect of Surface Sterilizing Agents on In Vitro Culture Establishment of Guava (Psidum guajava L.). J. Appl.Hort, 2(2): 94-95.
Mihaljević, I., Dugalić, K., Tomaš, V., Viljevac, M., Pranjić, A., Čmelik, Z., Puškar, B., and Jurković, Z. 2013. In Vitro Sterilization Procedures for Micropropagation of ‘oblačinska’ Sour Cherry. Journal of Agricultural Sciences, 58(2): 117-126.
Minipara, D., Hareshkumar, D., Ghansyam, P., Subhash, N., and Sushil, K. (2021). Identification of Best Surface Sterilization Treatment and Control of Endophtic Bacterial Contamination in Annona squamosal L. International Journal of Plant & Soil Science, 29(6): 1-10.
Natasha, K., and Restiani, R., 2019. Optimasi Sterilisasi Eksplan pada Kultur In Vitro Ginseng Jawa (Talium paniculatum). Prosiding Symbion (Symposium on Biology Education), Prodi Pendidikan Biologi, FKIP, Universitas Ahmad Dahlan (30 Agustus 2019). 87-95.
Neliyati, N., Lizawati, L., and Zulkarnain, Z. (2019). The Evaluation of Sterilization Protocol for Sprout Explants in Oil Palm Elaeis guineensis Jacq. Tissue Culture. Journal of Physics: Conference Series, 1402: 1-6.
Oksana, Elfi, R., & Syamsul. (2011). Peranan Berbagai Macam Media Tumbuh Bagi Pertumbuhan Stek Daun Jeruk JC (Japansche Citroen) dengan Beberapa Konsentrasi BAP. Jurnal Penelitian, 11(1): 1-8.
Rasud, Y., Ulfah, S., dan Baharia. (2015). Pertumbuhan Jeruk Manis (Citrus sinensis L.) dengan Penambahan Berbagai Konsentrasi Sitokinin Secara In Vitro. Jurnal Agroland, 3(22): 197-204.
Shukla, S., Singh, M., Kumar, A., Rajbhar, Y., Kumar, M., and Singh, S. P. (2019). Effects of Surface Sterilization Agents Under In Vitro Culture of Banana (Musa paradisiaca L.) Variety “Udhayam”. Journal of Pharmacognosy and Phytochemistry, 8(1): 843-846.
Sugiyatno, A., 2017. Potensi Penggunaan Beberapa Varietas Batang Bawah Sebagai Interstock untuk Memacu Pertumbuhan Benih Jeruk. Balai Penelitian Tanaman Jeruk dan Buah Subtropika. Malang.
Surya, M. I., dan Ismaini, L. (2021). Perbandingan Metode Sterilisasi untuk Perbanyakan Rubus rosifolius Secara In Vitro. Al-Kauniyah: Jurnal Biologi, 14(1): 127-137.
Wahyono, N. D., Hasanah, N., and Nurprahastani, N. (2021). Optimization of Sterilization Techniques and Effects of Coconut Water for the Induction of Shoots of Stevia (Stevia rebaudiana Bertoni). Proceedings of The 3rd International Conference on Food and Agriculture, 2021.
Yao, N., Bi, F., Wang, Y., Jr, G. F., Hyuk Suh, J., Tang, X., Zhang, Y., and Gmitter Jr, F. G. (2021). Metabolomic Analysis Provides New Insight Into Tolerance of Huanglongbing in Citrus. Front. Plant Sci, 12: 710598.
Published
2022-11-30
How to Cite
TUWO, MUSTIKA et al.
Pengaruh Metode Sterilisasi Permukaan terhadap Kultur Biji Jeruk Japansche Citroen (JC) Citrus limonia Osbeck.
Agrotrop : Journal on Agriculture Science, [S.l.], v. 12, n. 2, p. 219-229, nov. 2022.
ISSN 2654-4008.
Available at: <https://ojs.unud.ac.id/index.php/agrotrop/article/view/94523>. Date accessed: 03 july 2024.
doi: https://doi.org/10.24843/AJoAS.2022.v12.i02.p04.
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