Perkecambahan Asimbiotik Biji Anggrek Dendrobium bicaudatum pada Media dengan Penambahan Ekstrak Tomat secara In Vitro

  • NI WAYAN SURIANTARI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. P.B. Sudirman Denpasar Bali 8023
  • IDA AYU PUTRI DARMAWATI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. P.B. Sudirman Denpasar Bali 8023
  • HESTIN YUSWANTI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. P.B. Sudirman Denpasar Bali 8023

Abstract

Asymbiotic Germination of Dendrobium bicaudatum Orchid Seeds on Media with In Vitro Addition of Tomato Extract. Dendrobium bicaudatum is a natural orchid that needs to be preserved. Orchid seeds propagation naturally (symbiotic) has a low success rate. Orchid seeds do not have an endosperm. Asymbiotic germination with in vitro culture techniques can be an alternative solution for the propagation of orchid seeds. This study aimed to determine the effect of giving tomato extract on modified media on the asymbiotic germination of Dendrobium bicaudatum orchid seeds. This research used the Completely Randomized Design with five treatments of tomato extract concentration, namely 0%, 5%, 10%, 15%, 20%, with 4 replications. The variables observed in this study were the time and percentage of phase 1; time and percentage of phase 2; time and percentage of phase 3; time and percentage of phase 4; time and percentage of phase 5; and time and percentage of phase 6. The results showed that the tomato extract treatment had a significant or very significant effect on the germination of Dendrobium bicaudatum orchid seeds and the best treatment for the fastest and the highest percentage of germination from protocorm to First Leaf Primodium was the addition of tomato extract with a concentration of 15% or 20%, and a concentration of 0% tomato extract for the early stages of swelling of the embryo.

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Published
2022-05-31
How to Cite
SURIANTARI, NI WAYAN; DARMAWATI, IDA AYU PUTRI; YUSWANTI, HESTIN. Perkecambahan Asimbiotik Biji Anggrek Dendrobium bicaudatum pada Media dengan Penambahan Ekstrak Tomat secara In Vitro. Agrotrop : Journal on Agriculture Science, [S.l.], v. 12, n. 1, p. 49-62, may 2022. ISSN 2654-4008. Available at: <https://ojs.unud.ac.id/index.php/agrotrop/article/view/87075>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.24843/AJoAS.2022.v12.i01.p05.
Section
Articles