Perkecambahan Asimbiotik Biji Anggrek Dendrobium bicaudatum pada Media dengan Penambahan Ekstrak Tomat secara In Vitro

NI WAYAN SURIANTARI; IDA AYU PUTRI DARMAWATI; HESTIN YUSWANTI

doi:10.24843/AJoAS.2022.v12.i01.p05

NI WAYAN SURIANTARI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. P.B. Sudirman Denpasar Bali 8023
IDA AYU PUTRI DARMAWATI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. P.B. Sudirman Denpasar Bali 8023
HESTIN YUSWANTI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Jl. P.B. Sudirman Denpasar Bali 8023

DOI: https://doi.org/10.24843/AJoAS.2022.v12.i01.p05

Abstract

Asymbiotic Germination of Dendrobium bicaudatum Orchid Seeds on Media with In Vitro Addition of Tomato Extract. Dendrobium bicaudatum is a natural orchid that needs to be preserved. Orchid seeds propagation naturally (symbiotic) has a low success rate. Orchid seeds do not have an endosperm. Asymbiotic germination with in vitro culture techniques can be an alternative solution for the propagation of orchid seeds. This study aimed to determine the effect of giving tomato extract on modified media on the asymbiotic germination of Dendrobium bicaudatum orchid seeds. This research used the Completely Randomized Design with five treatments of tomato extract concentration, namely 0%, 5%, 10%, 15%, 20%, with 4 replications. The variables observed in this study were the time and percentage of phase 1; time and percentage of phase 2; time and percentage of phase 3; time and percentage of phase 4; time and percentage of phase 5; and time and percentage of phase 6. The results showed that the tomato extract treatment had a significant or very significant effect on the germination of Dendrobium bicaudatum orchid seeds and the best treatment for the fastest and the highest percentage of germination from protocorm to First Leaf Primodium was the addition of tomato extract with a concentration of 15% or 20%, and a concentration of 0% tomato extract for the early stages of swelling of the embryo.

Downloads

Download data is not yet available.

References

Darmawati, I.A.P., I.A. Astarini, H. Yuswanti, and Y. Fitriani. (2021). Pollination Compatibility of Dendrobium spp. Orchids from Bali, Indonesia, and the Effects of Adding Organic Matters on Seed Germination Under In Vitro Culture. Biodiversitas, 22(5): 2554-2559.
Dwiyani, R., Purwantoro, A., Indrianto, A., dan Semiarti, E. (2009). Peningkatan Kecepatan Pertumbuhan Embrio Aggrek Vanda tricolor Lindl. pada Medium Diperkaya dengan Ekstrak Tomat. Prosiding Seminar Nasional Biologi XX dan Kongres PBI XIV UIN Maliki Malang 24-25 Juli 2009. 590 – 596.
Dwiyani, R. (2013). Perkecambahan Biji dan Pertumbuhan Protokorm Anggrek dari Buah dengan Umur yang Berbeda pada Media Kultur yang Diperkaya dengan Ekstrak Tomat. Jurnal Hortikultura Indonesia 4(2): 90-93.
Dwiyani, R., H. Yuswanti, I.A.P. Darmawati, K. Suada, and N.N.A. Mayadewi. (2015). In vitro Germination and Its Subsequent Growth of an Orchid of Vanda tricolor Lindl. var. suavis from Bali on Complex Additives Enriched Medium. Agrivita, 37(2): 144-150.
Dwiyani, R. (2015). Kultur Jaringan Tanaman. Pelawa Sari. Denpasar Barat.
Goncalves, L.M., E.C. Prizoa, M.A.M. Gutierre, C.A. Mangolin, and M.F.P.S. Machado. (2012). Use of complek supplements and light-differential effects for micropropagation of Hadrolaelia purpurata (= Laelia purpurata) and Encyclia randii orchid. Acta Scientiarum. Agronomy, 34(4): 459-463.
Mulyono, D. (2010). Pengaruh Zat Pengatur Tumbuh Auksin: Indole Butric Acid (IBA) dan sitokinin: Benzil Amino Purine (BAP) dan Kinetin dalam Elongasi Pertunasan Gaharu (Aquilaria beccariana). Jurnal Sains dan Teknolohi Indonesia, 12(1): 1-17.
Prasetya, W., I. Yulianah, dan S.L. Purnamaningsih. (2017). Pengaruh Teknik Ekstraksi dan Varietas terhadap Viabilitas Benih Tomat (Lycopersicum esculentum L.). Jurnal Produksi Tanaman, 5(2): 257-264.
Saputri, W. (2015). Respon Pertumbuhan Anggrek Hitam (Coelogyne pandurata Lindl.) Secara In vitro dengan Penambahan Ekstrak Taoge dan Benzyl Amino Purine (BAP). Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Tanjungpura. Pontianak.
Setiari, N. A. Purwantoro, S. Moeljopawiro, and E. Semiarti. (2016). Peptone and Tomato Extract Induced Early Stage of Embryo Development of Dendrobium Phalaenopsis Orchid. Jurnal of Tropical Biodiversity and Biotechnology, 1(2): 77-84.
Sugiyarto, L., S. Umniyatie, dan V. Henuhili. (2016). Keanekaragaman Anggrek Alam dan Keberadaan Mikoriza Anggrek di Dusun Turgo Pakem, Sleman Yogyakartai. Jurnal Sains Dasar, 5(2): 71-80.
Utami, A.L., A. Romeida, dan D.W. Ganefianti. (2014). Analisis Keragaman 20 Aksesi Anggrek Alam. Akta Agrosia, 17(2): 151-166.
Wahyudiningsih, T.S., Y. Jagau, dan N. Ravenska. (2018). Konservasi Coelogyne pandurata Lindh. di Kalimantan Tengah: Karakter, Morfologi, Propagasi In vitro, dan Pelestarian Berbasis Komunitas Lokal. Jurnal Pengelolaan Lingkungan Berkelanjutan, 2(2): 125-139.
Wijayani, Y., Solichatun, dan W. Mudyantini. (2007). Pertumbuhan Tunas danStruktur Anatomi Protokorm Like Body Anggrek Grammatophyllum scriptum (Lindl.) BI. dengan Pemberian Kinetin dan NAA. Bioteknologi, 4(2): 33-40.
Yeung, E.C. (2017). A Perspective on Orchid Seed and Protocorm Development. Botanical Studies, 58(33): 1-14.