Perbanyakan Tanaman Jeruk Siam (Citrus nobilis L.) dengan Teknik Kultur in vitro Menggunakan Biji Tanaman Terinfeksi Penyakit Citrus Vein Phloem Degeneration (CVPD)

  • BAYU ARYA BAGASKARA Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
  • I GEDE PUTU WIRAWAN Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
  • MADE SRITAMIN Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
  • I GUSTI AYU DIAH YUNITI Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Abstract

Citrus (Citrus nobilis L.) Propagation Through Culture In Vitro Using Seed From Infected Plants Of Citrus Vein Phloem Degeneration (CVPD) Disease. The sample was taken in Belancan Village, Kintamani District, Bangli Regency, and invitro culture was conducted in UPT. Genetics Resource and Molecular Biology Laboratory in February to June 2018. This research purposes were to get the new citrus plant from citrus seed infected of CVPD disease explants with culture in vitro and found the presence of the bacterium Liberobacter asiaticus in results of citrus plants culture in vitro. The sampling method is using plants appearance. Obtained sample 1 is a plant that shows severe symptoms of CVPD disease and sample 2 plants that show no symptoms of CVPD disease. Seed Sample is cultured with MS0 medium for 8 WAP then proceed to acclimatization. The results of this research showed that the seed explants from both samples were able to grow well and did not have the characteristic of CVPD disease, but explant seed sample 2 in vitro culture result showed that the bacterial DNA bacterium Liberobacter asiaticus pathogen CVPD disease does exist in length 1160bp, so it cannot be expected to produce CVPD-free crops.

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References

Dwiyani, R. 2015. Kultur Jaringan Tanaman. Pelawa Sari. Denpasar Barat. 75.
Hung, T.H., Wu, M.L., And Su, H.J. 2000. Detection of fastidious bacteria causing citruss greening disease by nonradioactive DNA probes.
Irmawati. 2003. Perubahan Keragaman Genetika Ikan Kerapu Tikus Generasi Pertama Pada Stok Hatchery. Thesis. Bogor: IPB.
Melani, R. 2018. Deteksi Penyakit Citrus Vein Phloem Degeneration
(CVPD) Dengan Teknik Polymerase Chain Reaction(PCR) pada Daun Tanaman Jeruk Yang MemilikiPola Gejala Klorosis Berbeda. E-Jurnal Agroekoteknologi Tropika. 7(2). 164-173.
Sandrine, J., J.M. Bove, M. Garnier. 1996. PCR detection of two candidates Liberobacter spesies assosiated with greening disease of citrus. Moleculeran Celluler Probes. 10: 42.
Sritamin, M. 2007. Akumulasi Protein Spesifik 16 kDa (PS16) pada Daun Jeruk Terinfeksi Penyakit Citrus Vein Phloe Degeneration (CVPD). Disertasi (sudah dipublikasi) Universitas Brawijaya Malang.
Suamba, I. W., I. G. P. Wirawan, Dan Wayan Adiartayasa. 2014. Isolasi dan Identifikasi Fungi Mikoriza Arbuskular (Fma)secara Mikroskopis pada Rhizosfer Tanaman Jeruk (Citrus sp.)di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar. E-Jurnal Agroekoteknologi Tropika,2(4): 201-208.
Suyamto, Supriyanto A, Agustian A, Triwiratno A, Dan Winarno M. 2005. Prospek dan Arah Pengembangan Agribisnis Jeruk. Badan Penelitian dan Pengembangan Pertanian, Departemen Pertanian, Jakarta.
Wirawan, I.G.P., N. Arya., S. Subandiyah. 1998. Isolasi Loci Resisten Terhadap CVPD dengan Metode Transformasi Menggunakan Agrobacteriumtumefaciens. Laporan Riset Unggulan Terpadu V. Kantor Menteri Negara Riset Teknologi. Dewan Riset Nasional. Jakarta.
Wirawan, I.G.P. 2000. Isolasi Resisten terhadap CVPD (Citrus Vein Phloem Degeneration) dengan Metode Transformasi Menggunakan Agrobacterium tumefaciens. Laporan Riset Unggulan Terpadu V. Denpasar: Universitas Udayana.
Wirawan, I.G.P., L. Sulistyowati, dan I.N. Wijaya. 2003. Mekanisme Tingkat Molekul Infeksi Penyakit CVPD pada Tanaman Jeruk dan Peran Diaphorinacitri Kuw. sebagai Serangga Vektor. Denpasar. Lemlit. Universitas Udayana.
Published
2019-07-19
How to Cite
BAGASKARA, BAYU ARYA et al. Perbanyakan Tanaman Jeruk Siam (Citrus nobilis L.) dengan Teknik Kultur in vitro Menggunakan Biji Tanaman Terinfeksi Penyakit Citrus Vein Phloem Degeneration (CVPD). Agrotrop : Journal on Agriculture Science, [S.l.], v. 8, n. 2, p. 179-188, july 2019. ISSN 2654-4008. Available at: <https://ojs.unud.ac.id/index.php/agrotrop/article/view/51174>. Date accessed: 09 oct. 2024. doi: https://doi.org/10.24843/AJoAS.2018.v08.i02.p09.
Section
Articles