Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii

  • EDY SETITI WIDA UTAMI Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga
  • SUCIPTO HARIYANTO Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga

Abstract

Optimalization protoplast isolation of the leaves mesophyll orchid Paraphalaenopsis layockii. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii. The factorial experiment consisted of two factors that was arranged by completely randomized design (CRD) with ten replications. The first factor was concentrations of sucrose which consists of three levels namely 0.4 M, 0.5 M and 0.6 M. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. The results showed that there was effect of treatment for all variables observed. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.6 M sucrose and 4 hours of incubation time.

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How to Cite
UTAMI, EDY SETITI WIDA; HARIYANTO, SUCIPTO. Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii. Agrotrop : Journal on Agriculture Science, [S.l.], v. 5, n. 1, p. 21-29, jan. 2016. ISSN 2654-4008. Available at: <https://ojs.unud.ac.id/index.php/agrotrop/article/view/18370>. Date accessed: 05 dec. 2020.
Section
Articles

Keywords

protoplast isolation; leaves mesophyll; Paraphalaenopsis layockii