Pengaruh Kombinasi Naphthalene Acetic Acid (NAA) –Benzyl Amino Purine (BAP) dan Jenis Eksplan pada Mikropropagasi Anggrek Vanda tricolor Lindl. var. suavis

  • AYU INDAH WIDAYANTI Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
  • RINDANG DWIYANI Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
  • HESTIN YUSWANTI Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Abstract

The Effect of Combination of BAP-NAA and Explant Types on Micropropagation of Orchidof Vanda tricolor Lindl. var. suavis

The objectives of the research was to investigate the effect ofcombination of NAA–BAP, type of explant and their interactions on shoot initiation in micropropagationof Vanda tricolor L. var. suavis. The factorial experiment consisted of two factors that was arrangedby completely randomized design (CRD) with three replications. The first factor was combination ofNAA-BAP i.e. M0= MS medium without any plant growth regulator; M1= MS + 0,1 mgL-1 NAA +0 mgL-1 BAP; M2= MS + 0,1 mgL-1 NAA + 0,1 mgL-1 BAP; M3= MS + 0,1 mgL-1 NAA + 1 mgL-1 BAP ; and M4= MS + 0,1 mgL-1 NAA + 10 mgL-1 BAP. The second factor was explant type i.e.shoot tip (B1) and shoot base (B2). The results showed that there was no interaction between factorsfor all variable observed. Medium M0 (MS medium without any plat growth regulator) and explant ofB1 (shoot tip) gave the highest number of the percentage of explants producing shoots and the highestnumber of shoots per explant.

Published
2014-05-01
How to Cite
WIDAYANTI, AYU INDAH; DWIYANI, RINDANG; YUSWANTI, HESTIN. Pengaruh Kombinasi Naphthalene Acetic Acid (NAA) –Benzyl Amino Purine (BAP) dan Jenis Eksplan pada Mikropropagasi Anggrek Vanda tricolor Lindl. var. suavis. Agrotrop : Journal on Agriculture Science, [S.l.], v. 4, n. 1, p. 13-18, may 2014. ISSN 2654-4008. Available at: <https://ojs.unud.ac.id/index.php/agrotrop/article/view/13624>. Date accessed: 19 sep. 2019.
Section
Articles

Keywords

micropropagation; NAA-BAP; V. tricolor Lindl var. suavis; shoot initiation, explant types