Induksi Mutasi Tanaman Cabai Merah (Capsicum Annuum L.) dengan Ethyl Methanesulfonate pada Berbagai Tingkat Waktu Perendaman

  • I MADE AGUS WIARTANA Program Studi Magister Ilmu Biologi, Program Pascasarjana Universitas Udayana, Bali
  • MADE PHARMAWATI Jurusan Biologi, Fakultas MIPA Universitas Udayana, Bali
  • I KETUT SUADA Jurusan Agroekoteknologi, Fakultas Pertanian Universitas Udayana, Bali

Abstract

Induction of Mutation of Red Chili (Capsicum Annuum L.) Using Ethyl Methanesulfonate at Several Soaking Periods.

One way to increase genetic variation is through induced mutation usingchemical mutagen. Ethyl methanesulfonate (EMS) is a chemical compound that can cause mutationsand commonly used in plant. In this study seeds of red chili were treated using EMS 1% through seedsoaking. Seeds of red chili were soaked with EMS 1% in phosphate buffer pH 7 for 6, 9, 12 and 15hours at room temperature. As control, seeds were soaked in phosphate buffer pH 7. This study aimsto evaluate, physiological and reproductive characters of plants after treated with EMS. Experimentwas conducted in an open field with 5 replicates for each treatment. Results showed that concentrationof chlorophyll a, b and total chlorophyll increased in plants derived from seed treated with EMS 1% for9 hours compared to control and other soaking periods. Soaking seeds with EMS 1% for 12 hoursincreased viability of pollen compared to control and other treatments. The first time of floweringoccurred earlier at 6 and 9 hours soaking period.

Published
2014-05-01
How to Cite
WIARTANA, I MADE AGUS; PHARMAWATI, MADE; SUADA, I KETUT. Induksi Mutasi Tanaman Cabai Merah (Capsicum Annuum L.) dengan Ethyl Methanesulfonate pada Berbagai Tingkat Waktu Perendaman. Agrotrop : Journal on Agriculture Science, [S.l.], v. 4, n. 1, p. 7-12, may 2014. ISSN 2654-4008. Available at: <https://ojs.unud.ac.id/index.php/agrotrop/article/view/13622>. Date accessed: 21 sep. 2019.
Section
Articles

Keywords

Ethyl Methanesulfonate; morphology; physiology; red chili; reproductive; soaking period