Kandungan Fitokimia Kulit Buah Jeruk Siam (Citrus nobilis Lour. var. microcarpa) Sehat dan Berpenyakit CVPD di Desa Catur, Kintamani Menggunakan GC-MS (Gas Chromatography-Mass Spectrometry)
Abstract
Phytochemical Content of Healthy and Infected by Citrus Vein Phloem Degeneration Siam Citrus (Citrus nobilis Lour. var. microcarpa) Peels in Catur Village, Kintamani Using GC-MS (Gas Chromatography-Mass Spectrometry). Siam citrus (Citrus nobilis Lour. var. microcarpa) is one of the potential plants because it is widely developed in Bali and its taste is favored by consumers. The productivity of Siam citrus plants in Indonesia is still inadequate because it is often infected by Citrus Vein Phloem Degeneration (CVPD) caused by the Literobacter bacteria. The purpose of this study is to determine the difference content of phytochemical compounds of Healthy Siam citrus and Infected by Citrus Vein Phloem Degeneration citrus peel extract and the use of phytochemical compounds contained. Phytochemical analysis was performed with Gas Chromatograhy- Mass Spectrometry (GC-MS). The GC-MS analysis result showed that there was difference phytochemical compounds of Healthy Siam citrus and Infected by Citrus Vein Phloem Degeneration citrus peel extract. Healthy Siam citrus peel contain compounds such as Limonene, Cholestan-3-ol, 4',5,7,8-Tetramethoxyflavone, Beta-Elemene,5-Hydroxymethylfurfural, Octanal, Humulene, Noanal, Decanal, Perillartine, Citronellal, and Carveol. While the siam citrus peel infected by Citrus Vein Phloem Degeneration contains compounds such as Glycerin, 1,2- Cyclohexanediol,1-methyl-4-(1-methylethenyl), Benzoic Acid, Benzofuran, 2,3-dihydro-, 3,4-Altrosan, 2-Propenoic acid, 3-(4-hydroxy-3-methoxyphenyl), 5- Desmethysinensetin, Ferullic Acid, Furan, Itaconic Anhydride, Cyclohexane, 1-ethenyl-1-methyl-2,4-bis (1-methylethenyl)-, L-Carvone, n-Hexadecanoic acid. The presence of differences in secondary metabolites is caused by plants when exposed to biotic stress or disease, plants will produce more phytochemicals that used for mechanical defense of plant in nature.
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References
Badan Pusat Statistik. 2021. “Produksi jeruk di Bali tahun 2019-2021” diakses dari bali.bps.go.id. diakses pada 16 Agustus 2022
[Balitjestro] Badan Penelitian Tanaman Jeruk dan Buah Subtropika. 2014. Tips Memilih Varietas Sebelum Menanam Jeruk. http//balitjestro.litbang.pertanian.go.id/tips-memilih-varietas-sebelum-memanam-jeruk/ diakses pada 15 Agustus 2022
Bouyahya, A.; Mechchate, H.; Benali, T.; Ghchime, R.; Charfi, S.; Balahbib, A.; Burkov, P.; Shariati, A. M.; Lorenzo, J. M.; El Omari, N. Health Benefits and Pharmacological Properties of Carvone. Biomolecules. 2021, 11 (12), 1803. DOI: 10.3390/biom11121803
Callahan, FE; Johnie N. Jenkins; Roy G. Creech; Gary W. Lawrence. 1997. Integrated Pest Management Research Unit. Crop Science Research Laboratory, Missisipi State.
Departemen Kesehatan RI. 2014. Peraturan Menteri Kesehatan Republik Indonesia Nomor 5. Jakarta: Depkes RI, p441-448.
Departemen Pertanian. 2012. Kajian Umum Mengenai Jeruk. Available at: http://ditlin.hortikultura.go.id/jeruk_cvpd/jeruk01.html Diakses pada 6 Agustus 2022
Direktorat Jendral Pengawasan Obat dan makanan 1987. Analisis Obat tradisional. Jilid 1. Departemen Kesehatan RI, Jakarta
Garnier M., Bove J.M., 1990. Distribution of the huanglongbing (greening) litobacter species in fifteen African ans Asian countries. Proceedings of 13th Conference IOCV, IOCV, Riverside 1990, 388-391
Kumar N, Pruthi V. Potential applications of ferulic acid from natural sources. Biotechnol Rep (Amst). 2014 Sep 16;4:86-93. doi: 10.1016/j.btre.2014.09.002. PMID: 28626667; PMCID: PMC5466124.
Lygin Anatoly V., Li Suxian, Vittal Ramya, holm Jack M., Hartman Glen L., and Lozovaya Vera V. 2009. The importance of phenolic metabolism to limit the growth of Phakopsora pachyrhizi. Phytopathology 99(12): 1412-1420
Mohammad, M. K. T, Md.MM, Raunak. J, Artho. B, A.K.M.A Hoque. 2021. Identification of citrus greening based on visual symptomps; A grower’s diagnostic toolkit. Journal of Heliyon. Vol 8
Meitayani, Ni Putu Swari ; Adiartayasa, Wayan; WIjaya, I Nyoman. Deteksi Penyakit Citrus Vein Phloem Degeneration (CVPD) dengan Teknik Polymerase Chain Reaction (PCR) pada Tanaman Jeruk di Bali. Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology), [S.l.], may 2014. ISSN 2301-6515. Available at:
Najwa A. 2022. Analisis Fitokimia Ekstrak Daun Tanaman Kemangi Ocimum basilicum L. dan Ocimum citriodorum Lour Secara GC-MS (Gas Chromatography- Mass Spectrometry) Skripsi. Fakultas Pertanian, Universitas Udayana, Bukit Jimbaran.
Nakashima, K., M. Prommintara, Y. Ohtsu, T.Kano, J. Imada and M. Koizumi. 1996. Detection of 16 A rDNA of Thai isolates of bacterium like organism associated with greening disease of citrus. JIRCAS J. 3:1-8.
National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 72344, Nobiletin. Retrieved January 11, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/Nobiletin.
National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 332, 2-Methoxy-4-vinylphenol. Retrieved January 11, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/2-Methoxy-4-vinylphenol.
Wirawan, I G.P. 2003. Mekanisme Infeksi Penyakit CVPD (Citrus Vein Phloem Degeneration) pada Tanaman Jeruk. Bahan Kuliah Program Pscasarjana, Program Studi Bioteknologi Pertanian. Denpasar: Universitas Udayana
Youngji Jung, Shinai Choi, Keum-Soon Oh, Namkyu Sun. (2022) Exposure assessment of benzoic acid from processed foods in South Korea. Food Additives & Contaminants: Part A 39:1, pages 14-23
