Perbandingan Berat Molekul Protein Total Tanaman Selada (Lactuca sativa L.) Hasil Plant Factory dan Ditanam di Tanah Menggunakan SDS-PAGE

Muhamad Farrel Rizki; I Gede Putu Wirawan; I Ketut Suada

doi:10.24843/AJoAS.2024.v14.i02.p13

Muhamad Farrel Rizki Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Jl. PB. Sudirman Denpasar Bali 80231, Indonesia
I Gede Putu Wirawan Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Jl. PB. Sudirman Denpasar Bali 80231, Indonesia
I Ketut Suada Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Jl. PB. Sudirman Denpasar Bali 80231, Indonesia

DOI: https://doi.org/10.24843/AJoAS.2024.v14.i02.p13

Abstract

Comparison of Total Protein Molecular Weight of Lettuce (Lactuca Sativa L.) Plant Factory and Planted in the Ground Using SDS-PAGE. Lettuce (Lactuca sativa L.) is a plant that contains nutrients and protein that are good for human needs. Lettuce is often found in Indonesian agriculture because lettuce has growing conditions that are very suitable for the Indonesian climate. The purpose of this study was to determine the comparison of protein profiles of lettuce plants grown on the ground with those grown using the hydroponic plant factory system. This research was conducted from May to June 2023 at the Laboratory of PT Genetika Science Indonesia, Tangerang City. This research stage consists of sampling, protein extraction, electrophoresis, and data analysis. Comparisons were made by comparing the two sample data that had been carried out by electrophoresis by looking at the molecular weights of each sample. Plants grown in the soil had higher molecular weights than those grown in the plant factory and each had 12 different proteins. Both samples produced two other same protein bands of 69.65 kDa and 11.97 kDa.

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