ISOLATION OF Deoxyribonucleic acid (DNA) USING COMMERCIAL KITS AND CONVENTIONAL METHODS

Abstract

The livestock sector, as a provider of animal protein in developing countries like Indonesia, faces challenges in enhancing productivity through breeding. Advances in molecular biology technology have enabled accelerated breeding using marker-assisted selection (MAS). One crucial step in this technology to determine markers is DNA isolation. This study aims to compare DNA isolation methods in Taro cattle using commercial kit A, commercial kit B, and Chelex. Observation parameters include DNA concentration and purity measured with a nanodrop spectrophotometer. The study shows that the highest DNA concentration was obtained using the Chelex method at 255.62 ng/?L, followed by commercial kit B at 6.19 ng/?L, and commercial kit A at 3.27 ng/?L. In terms of DNA purity, the most pure DNA samples were produced by commercial kit A with three samples, followed by commercial kit B with two samples, while the Chelex method did not yield any pure DNA samples. The shortest DNA isolation time was achieved by commercial kit B, followed by commercial kit A, and the Chelex method. In terms of cost efficiency, the Chelex method is the most economical compared to commercial kit A and commercial kit B.