EFEKTIVITAS EKSTRAK BUNGA KENANGA (Cananga odorata) UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Colletotrichum acutatum PENYEBAB PENYAKIT ANTRAKNOSA PADA TANAMAN CABAI BESAR (Capsicum annum)
Abstrak
Colletotrichum acutatum is a pathogenic fungal that causes anthracnose disease in cultivated plants, one of them is chili. The anthracnose disease commonly controlled by using synthetic pesticides. The continuous use of synthetic pesticides can cause adverse side effects, so the alternative compound was need to replaced by plant-based fungicides. This study aimed to determine the effectiveness of cananga flower extract in inhibiting the growth of Colletotrichum acutatum and to determine the group of bioactive compounds in cananga flower extract. The study used a Completely Randomized Design (CRD) with seven treatments consisting of P0: negative control, P1: positive control, and with different consentration of cananga extracts at P2: 1%(w/v), P3: 2%(w/v), P4: 3%(w/v), P5: 4%(w/v), and P6: 5%(w/v) with each treatment repeated 4 times. The parameters observed were zone of inhibition, colony diameter, spores density, and phytochemical test. The results of the diffusion well test showed that largest inhibition zone at concentration of 5% (P6) by 10,05 mm and smallest inhibition zone at concentration 1% (P2) by 7,53 mm. The result of the colony test showed that smallest colony diameter on the 10th day was at concetration of 3% (P4) by 7,09 cm and the largest colony diameter was at concentration of 1% (P2) by 8,46 cm. The result of the spore density test showed that the highest density at concentration 1% (P3) by 3,24×104 spores/mL and the smallest spore number at concentration 3% (P4) by 2,36×104 spores/mL. The group of active compunds found in cananga flower extract includes alkaloid, steroid, flavonoid, phenolic, saponin, and tanin.
##plugins.generic.usageStats.downloads##
Referensi
Angelina, S., I. N. Suartha dan A. L. T. Rompis. 2018. Efektivitas Ekstrak daun Sirsak Terhadap Jamur Microsporum gypseum Penyebab Dermatitis Kompleks Pada Anjing. Buletin Veteriner Udayana. 12(2): 155-160.
Awaludin, M. A., Efri dan Sudiono. 2020. Pengaruh Ekstrak Daun Pepaya Terhadap Penyakit Antraknosa Pada Buah Pepaya. J. Agrotek Tropika. 8(3): 409-421.
Chand, R. R., A. D. Jokhan, R. D. Gopalan and T. Osborne. 2017. Antibacterial and Antifungal Activities of Essential Oils from Medical Plants Found in South Pacific. The South Pacific Journal of Natural and Applied Sciences. 35(1): 10-19
Damm, U., P. F. Cannon, J. H. C. Woundenberg and P. W. Crous. 2012. The Colletotrichum acutatum Species Complex. Studies in Micology. 73: 37-113.
Darmadi, A. A. K., I. K. Ginantra dan M. Joni. 2017. Uji Efektivitas Ekstrak Aseton Daun Kayu Manis (Cinnamomum burmanni Blume) Terhadap Jamur Fusarium solani Penyebab Penyakit Busuk Batang Pada Buah Naga (Hylocereus sp.) Secara in Vitro. Jurnal Metamorfosa. 4(1): 79-86.
Darmadi, A. A. K., S. K. Sudirga, N. L. Suriani dan I. G. A. S. Wahyuni. 2019. Antifungal Activities of Cinnamon Leaf Extracts Agaist Shigatoka Fungus (Pseudocercospora fijiensis). 6th International Conference on Sustainable Agriculture, Food and Energy. IOP. Conf. Series: Earth and Environmental Science. IOP Publishing.
Departemen Pertanian. 2004. Pola Produksi Hortikultura Direktorat Jendral Bina Produksi Hortikultura. Departemen Pertanian, RI. Jakarta.
Dusturia, N., S. R. Hikamah dan D. Sudiarti. 2016. Efektivitas Antibakteri Bunga Kenanga (Cananga odorata) dengan Metode Konvensional Terhadap Pertumbuhan Staphylococcus aureus. Bioshell. 5(1): 324-332.
Goldman, E. dan L. H. Green. 2009. Practical Handbook of Microbiology Edition 2nd. Taylor and Francis Group. New York.
Habazar, T. dan Yaherwandi. 2006. Pengendalian Hayati Hama dan Penyakit Tumbuhan. Andalas University Press. Padang.
Hersanti, F. L. dan I. Zulkarnaen. 2001. Pengujian Kemampuan Campuran Senyawa Benzothiadiazol 1% - Mankozeb 48% dalam Meningkatkan Ketahanan Cabai Merah Terhadap Penyakit Antraknosa. Prosiding Kongres Nasional XVI dan Seminar Hasil PFI. Bogor.
Kurnilia, K. W., S. K. Sudirga dan Y. Ramona. 2020. Potensi Minyak Atsiri Cananga odorata dan Cymbopon citratus dalam Menghambat Pertumbuhan Staphylococcus aureus ATCC 25923 dan Candida albicans ATCC 10231 Secara in Vitro. Jurnal Metamorfosa. 7(2): 191-198.
Kuspradini, H., A. S. Putri, E. Sukaton and T. Mitsunaga. 2015. Bioactivity of Essential Oils from Leaves of Dryopbalanops lanceolata, Cinnamomum burmanni, Cananga odorata, and Scorodocarpus borneensis. International Conference on Food, Agriculture, and Natural Resources, FANRes 2015. Universitas Jember. Jember.
Mengane, S. K. dan S. S. Kamble. 2014. Bioefficacy Of Plant Extracts n Fusarium oxisporum F. Sp. Cubense Causing Panama Wilt of Banana. IJPBS. 4(3): 24-27.
Morales, G., P. Sierra, A. Mancilla, A. Paredes, L. A. Loyola, O. Gallardo and J. Borques. 2003. Secondary Metabolites From our Medicinal Plant From Northern Chile: Antimicrobial activity and Biotoxicity Against Artemia salina. J. Chil.Chem Soc. 49(1): 44-49.
Putri, A. M., A. O. Muham, S. Anggraini, S. Maisarmah dan P. A. R. Yulis. 2020. Analisis Kualitatif Kandungan Bunga Kenanga (Cananga odorata) Secara Fitokimia Dengan Menggunakan Pelarut Etanol. Journal of Research Education Chemistry. 2(1): 43-48.
Rahmawati, F., M. Bintang dan I. M. Artika. 2017. Antibacterial Activity and Phytochemical Analysis of Geranium homeanum Turez Leaves. Current Biochemistry. 4(3): 13-22.
Rota, M. C., A. Herrera, R. M. Martinez, J.A. Sotomayor dan M. J. Jordan. 2008. Antimicrobial Activity and Chemical Composition Of Thymus vulgari, Thymus zygis and Thymus hyemalis Essential Oil. Food Control. 19: 681-687.
Santika. 2001. Agribisnis Cabai. Penebar Swadaya. Jakarta.
Sudirga, S. K. 2016. Isolasi dan Identifikasi Jamur Colletotrichum spp. Isolat PCS Penyebab Penyakit Antraknosa Pada Buah Cabai Besar (Capsicum annum L.) di Bali. Jurnal Metamorfosa. 3(1): 23-30.
Suharjo, R. dan T. N. Aeny. 2011. Eksplorasi Potensi Gulma Siam (Chromolaena odorata) Sebagai Biofungisida Pengendali Phytopthora palmivora) yang Diisolasi Dari Buah Kakao. Jurnal HPT Tropika. 11(2): 201-209.

This work is licensed under a Creative Commons Attribution 4.0 International License.



