Ability of Lactic Acid Bacteria Insulated from Mom's Milk in Reducing Blood Cholesterol Level of Wistar

Abstract

Bile salt hydrolase (BSH) is one of the enzymes produced by lactic acid bacteria. This enzyme has the
ability to lower cholesterol levels in vitro and in vivo. The lactic acid bacteria used in this study were
L. plantarum I A1, L. plantarum I A8, L. plantarum I B8, L.rhamnosus A6 and L. rhamnosus B10b which
a probiotic candidate isolated from breast milk. The purpose of this study was to determine the activity
of BSH and the ability to lower cholesterol both in vitro and in vivo. Three stages of experiment were
done. BSH activity and its ability in decreasing cholesterol in vitro were done for all strains of
Lactobacillus. The best strain is L. plantarum I B8 was continued for further experiment to determine
its ability to decrease blood cholesterol in in vivo using Wistar Rats.In vivo test results showed that L.
plantarum I B8 decreased total cholesterol, LDL, triglyceride, and HDL by 27%, 21%, 29% and 35%
respectively.