Profil Protein Stadium Sporozoit Eimeria tenella Isolat Yogyakarta Melalui Analisis Protein SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF Eimeria tenella ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) | Jurnal Veteriner

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Published Jul 20, 2013

Galuh Tresnani

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Joko Prastowo Wisnu Nurcahyo Budi Setiadi Daryono

Abstract

Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.

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TRESNANI, Galuh et al. Profil Protein Stadium Sporozoit Eimeria tenella Isolat Yogyakarta Melalui Analisis Protein SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF Eimeria tenella ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS). Jurnal Veteriner, [S.l.], v. 13, n. 2, p. 163-166, july 2013. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/5998>. Date accessed: 21 nov. 2024.

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Keywords

protein profile, SDS-PAGE, Eimeria tenella

Issue

Vol 13 No 2 (2012)

Section

Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License

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