Immunological Detection of Rabies Virus in Brain Tissues of Infected Dogs by Monoclonal Antibodies

  • Nyoman Mantik Astawa
  • Ida Bagus Kade Suardana
  • Luh Putu Agustini
  • Faiziah -

Abstract

In order to establish an immunological detection of rabies virus in tissues of infected dogs, monoclonalantibodies (mAbs) against rabies virus (RV) were produced. The mAbs were produced by fusion of mielomacells with the lymphocytes of mice immunized with RV. The mAbs produced were then characterized andused for the detection of rabies virus in brain tissues of infected dogs. Six mAbs designated CC6, EG4,DG10, BB12, CA9 dan EB5 were used in this study. In Western blotting test, some mAbs reacted with 66KDa which is the glycoprotein of the virus. In immunoperoxidase, 2 mAbs (CC6 and DG10) detected RVin the brain of infected dogs. By direct immunoflourescence, flourescence isotyocyanate (FITC) labelledDG10 mAbs detected RV in fresh and formaldehyde fixed brain tissues. RV was detected in 12 infecteddogs but not in normal uninfected dogs. In this study it was confirmed that rabies virus can be detected inthe brain tissues of infected dogs by monoclonal antibodies.

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Author Biographies

Nyoman Mantik Astawa
Virology Laboratory, Faculty of Veterinary Medicine, Udayana University,Jl. Sudirman, Denpasar, Bali, Indonesia 80232. Tel. 0361223791, Fax. 0361701808.
Ida Bagus Kade Suardana
Virology Laboratory, Faculty of Veterinary Medicine, Udayana University,Jl. Sudirman, Denpasar, Bali, Indonesia 80232. Tel. 0361223791, Fax. 0361701808.
Luh Putu Agustini
Virology Laboratory, Faculty of Veterinary Medicine, Udayana University,Jl. Sudirman, Denpasar, Bali, Indonesia 80232. Tel. 0361223791, Fax. 0361701808.
Faiziah -
Virology Laboratory, Faculty of Veterinary Medicine, Udayana University,Jl. Sudirman, Denpasar, Bali, Indonesia 80232. Tel. 0361223791, Fax. 0361701808.
How to Cite
MANTIK ASTAWA, Nyoman et al. Immunological Detection of Rabies Virus in Brain Tissues of Infected Dogs by Monoclonal Antibodies. Jurnal Veteriner, [S.l.], v. 11, n. 4, dec. 2010. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/3429>. Date accessed: 28 aug. 2026.
Citation Formats
Issue
Vol 11 No 4 (2010)
Section
Articles

Keywords

antibody, monoclonal, brain, rabies, dog
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License

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