Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR

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I Wayan Wirata Ida Ayu Sri Chandra Dewi I Gusti Ngurah Narendra Putra1, Ida Bagus Oka Winaya Ida Bagus Kade Suardana3, Tri Komala Sari I Nyoman Suartha I Gusti Ngurah Kade Mahardika

Abstract

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR

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How to Cite
WIRATA, I Wayan et al. Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR. Jurnal Veteriner, [S.l.], v. 11, n. 3, sep. 2010. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/3411>. Date accessed: 30 nov. 2020.
Keywords
classical swine fever virus, Bali, RT-PCR
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Articles

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