Main Article Content
Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.
Download data is not yet available.
How to Cite
SAILI, Takdir et al. PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN. Jurnal Veteriner, [S.l.], v. 10, n. 4, dec. 2009. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/3372>. Date accessed: 19 jan. 2021.
freeze drying, ram spermatozoa, plasma membrane, acrosome cap
This work is licensed under a Creative Commons Attribution 4.0 International License