PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN

Main Article Content

Takdir Saili I Ketut Mudite Adnyana Ronny Rachman Noor Mohamad Agus Setiadi Srihadi Agungpriyono Arief Boediono

Abstract

Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.

Downloads

Download data is not yet available.

Article Details

How to Cite
SAILI, Takdir et al. PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN. Jurnal Veteriner, [S.l.], v. 10, n. 4, dec. 2009. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/3368>. Date accessed: 05 nov. 2024.
Keywords
freeze drying, ram spermatozoa, plasma membrane, acrosome cap
Section
Articles

Most read articles by the same author(s)

1 2 3 > >>