Comparison of Cytokine Profile between Indonesian Thin-Tailed and Merino Sheep during A Primary Infection with Fasciola gigantica

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Ening Wiedosari David Piedrafita

Abstract

The aim of this study was to investigate the expression of cytokines profiles interferon-? (IFN-?),interleukin-5 (IL-5) and IL-10 in Rasciola gigantica resistant Indonesian thin-tailed (ITT) sheep comparedto susceptible merino sheep infected with Fasciola gigantica. A total of ten ITT and merino sheep wererandomly allocated into infected (n=5) and control (n=5) groups, sheep were infected with 250 viablemetacercariae of F. gigantica. The cytokines were determined by isolated mRNA from hepatic lymph nodeby semi-quantitative RT-PCR (Reverse Trancriptase -Polymerase Chain Reaction). The result showedITT sheep produced significantly higher IL-5 and 10 ( P<0,05 ) than merino sheep, while ITT sheepproduced less IFN-? ( P<0,05) than merino sheep at 10 weeks post infection. It could be concluded thatmerino sheep tend to develop T type 1 cells, while the ITT sheep tend to develop T type 2 cells whicheffectively killed F. gigantica.

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How to Cite
WIEDOSARI, Ening; PIEDRAFITA, David. Comparison of Cytokine Profile between Indonesian Thin-Tailed and Merino Sheep during A Primary Infection with Fasciola gigantica. Jurnal Veteriner, [S.l.], v. 13, n. 1, p. 20-25, oct. 2012. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/2133>. Date accessed: 08 aug. 2020.
Keywords
Fasciola gigantica; Indonesian thin-tailed sheep; merino sheep; IL-5; IL-10; IFN-?
Section
Articles