Keragaman Spesies dan Genetik Bakteri Staphylococcus pada Ikan Tuna dengan Analisis Sekuen 16s rRNA (SPECIES DIVERSITY AND GENETIC OF STAPHYLOCOCCUS BACTERIA IN TUNA FISH BY USING 16S rRNA SEQUENCE ANALYSIS) | Jurnal Veteriner

PDF

Published Oct 23, 2015

Putu Mei Purnama Dewi

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

I Nengah Kerta Besung I Gusti Ngurah Kade Mahardika

Abstract

Tuna industry belongs to high economic value fish of marine fisheries commodity. Bacteria found intuna might have impact on consumer health. The purpose of this research was to determine the geneticvariation of Staphylococcus in Kedonganan fish market, Kuta, Badung, Bali by sequence analysis of 16SrRNA. Staphylococcus bacteria was isolated from feces samples of 30 tuna. After identification with Gramstaining, Staphylococcus colonies were transferred to the medium chelex 10% for DNA extraction, 16SrRNA gene amplification by polymerase chain reaction (PCR), and electrophoresis. PCR results weresequenced, and the sequence obtained was edited using MEGA 5 and then BLAST was applied to confirmthe species. Genetic variation was determined by analysis of polymorphic sites with MEGA 5. The identifiedbacteria were Staphylococcus sciuri and Staphylococcus haemolyticus. Genetic distance of two isolates S.sciuri are close. The Other species that were identified were Enterococcus faecalis and Macrococcus caseolyticus.Conclusion on this research is Staphylococcus bacteria and two species S.sciuri have many variation.

Downloads

Download data is not yet available.

How to Cite

DEWI, Putu Mei Purnama; BESUNG, I Nengah Kerta; MAHARDIKA, I Gusti Ngurah Kade. Keragaman Spesies dan Genetik Bakteri Staphylococcus pada Ikan Tuna dengan Analisis Sekuen 16s rRNA (SPECIES DIVERSITY AND GENETIC OF STAPHYLOCOCCUS BACTERIA IN TUNA FISH BY USING 16S rRNA SEQUENCE ANALYSIS). Jurnal Veteriner, [S.l.], v. 16, n. 3, p. 409-415, oct. 2015. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/16256>. Date accessed: 25 apr. 2024.

ABNT APA BibTeX CBE EndNote - EndNote format (Macintosh & Windows) MLA ProCite - RIS format (Macintosh & Windows) RefWorks Reference Manager - RIS format (Windows only) Turabian

Keywords

Tuna Fish, Staphylococcus, 16S rRNA sequences, Kedonganan

Issue

Vol 16 No 3 (2015)

Section

Articles

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License

Most read articles by the same author(s)

I Nyoman Suartha, I Gusti Ngurah Kade Mahardika, Ida Ayu Sri Candra Dewi, Ni Ketut Dias Nursanty, Yosaphat L.S Kote, Anita Dwi Handayani, I Gusti Agung Ayu Suartini, THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER , Jurnal Veteriner: Vol 9 No 1 (2008)
Gusti Ayu Yuniati Kencana, Anak Agung Ayu Mirah Adi, Ida Bagus Komang Ardana, I Gusti Ngurah Kade Mahardika, Vaksin Gumboro Menyebabkan Imunosupresif pada Respons Primer Vaksin Penyakit Tetelo Ayam Pedaging , Jurnal Veteriner: Vol 12 No 4 (2011)
I Nyoman Suartha, I Wayan Wirata, I Gusti Ngurah Narendra Putra, Ni Made Ritha Krisna Dewi, I Made Suma Anthara, I Wayan Teguh Wibawan, I Gusti Ngurah Kade Mahardika, Vaksin Polivalen Untuk Mencegah Penyakit Flu Burung (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) , Jurnal Veteriner: Vol 13 No 2 (2012)
Gusti Ayu Yuniati Kencana, I Gusti Ngurah Kade Mahardika, Ida Bagus Kade Suardana, I Nyoman Mantik Astawa, Ni Made Krisna Dewi, Gusti Ngurah Narendra Putra, Pelacakan Kasus Flu Burung pada Ayam dengan Reverse Transcriptase Polymerase Chain Reaction* (DETECTION OF AVIAN INFLUENZA IN CHICKENS BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION) , Jurnal Veteriner: Vol 13 No 3 (2012)
I Gusti Ngurah Badiwangsa Temaja, I Nyoman Suartha, I Gusti Ngurah Kade Mahardika, Faktor-Faktor Risiko Tertular Flu Burung di Desa-Desa Kabupaten Klungkung, Bali (THE RISK FACTOR OF BIRD FLU CASES IN VILLAGES IN KLUNGKUNG REGENCY, BALI) , Jurnal Veteriner: Vol 14 No 2 (2013)
I Nyoman Dibia, Bambang Sumiarto, Heru Susetya, Anak Agung Gde Putra, I Gusti Ngurah Kade Mahardika, Helen Scott-Orr, Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) , Jurnal Veteriner: Vol 15 No 3 (2014)
Daud Steven Triyomi Hariyanto, I Gusti Ngurah Kade Mahardika, I Nengah Wandia, Keragaman Spesies Ikan Tuna di Pasar Ikan Kedonganan Bali dengan Analisis Sekuen Kontrol Daerah Mitokondria DNA (SPECIES DIVERSITY OF TUNA FISH USING MITOCHONDRIAL DNA CONTROL REGION SEQUENCE ANALYSIS AT KEDONGANAN FISH MARKET) , Jurnal Veteriner: Vol 16 No 3 (2015)
Ni Luh Made Ika Yulita Sari Hadiprata, I Made Bagus Arya Permana Ardiana Putra, I Gusti Ngurah Kade Mahardika, I Nengah Wandia, Tjokorda Sari Nindhia, Identifikasi Spesies Ikan Kerapu di Pasar Ikan Karangasem dan Kedonganan Bali Menggunakan DNA Mitokondria Gen 16s rRNA IDENTIFICATION OF GROUPER SPECIES USING 16S rRNA MITOCHONDRIAL DNA IN KARANGASEM AND KEDONGANAN FISH MARKET, BALI , Jurnal Veteriner: Vol 16 No 3 (2015)
Ni Ketut Suwiti, I Putu Suastika, Ida Bagus Ngurah Swacita, I Nengah Kerta Besung, Studi Histologi dan Histomorfometri Daging Sapi Bali dan Wagyu (HISTOLOGYCAL AND HISTOMORPHOMETRY STUDY OF BALI CATTLE AND WAGYU BEEF) , Jurnal Veteriner: Vol 16 No 3 (2015)
I Gusti Ayu Agung Suartini, Indrawati Sendow, Ni Luh Putu Agustini, Agik Suprayogi, I Wayan Teguh Wibawan, I Gusti Ngurah Kade Mahardika, Kinetika Immunoglobulin Kuning Telur Antiparvovirus Anjing Pada Anjing (KINETICS OF ANTICANINE PARVOVIRUS YOLK IMMUNOGLOBULIN IN DOGS) , Jurnal Veteriner: Vol 17 No 2 (2016)