Inactive Rabies Vaccine Stored at Room Temperature for 10 Hours Loses Its Ability to Stimulate Antibody Response in BALB/c Mice

Abstrak

Rabies vaccines are generally stored at 2-8°C to maintain antigen stability. However, most of Bali's mountainous and hilly areas often cause vaccines to be stored at room temperature or higher, which potentially damage the vaccine. One of the rabies vaccines used by the government is the inactivated rabies vaccine. This study was aimed to determine the effect of inactivated rabies vaccine storage time at room temperature to the immune response of BALB/c mice. This study used nine BALB/c mice that were divided into three different groups. Each group was induced with vaccines strored at room temperature (25-30°C) for 0 hours (P1), 6 hours (P2), or 10 hours (P3). Serum were collected before vaccination; and 2- and 4-weeks post vaccination. Antibodies were tested using an Enzyme-linked immunosorbent assay (ELISA) kit, and spleen samples were collected at week four for histological analysis with Hematoxylin Eosin staining. The results were analyzed using One-Way Analysis of Variance followed by Post Hoc-Bonferroni test. The histopathological examination of the spleen showed no differences between the three treatment groups. Our ELISA test showed that inactivated rabies vaccine stored for 10 hours at room temperature (25-30?) was not able to induce antibody response.