Keamanan Hair Tonic Ekstrak Etanol, Fraksi Etanol, dan Fraksi Kloroform-Metanol dari Kayu Secang (Caesalpinia sappan L.) dengan Metode Uji Iritasi Primer dan HET-CAM
Abstract
Hair tonic is a hair care cosmetic to reduce hair loss, stimulate, and increase hair volume. Secang wood (Caesalpinia sappan L.) has the potential to be developed as an active substance in hair tonic products because it contains flavonoids, polyphenols, including brazilin which are used to treat damage hair. To ensure the safety of hair tonic products, safety tests are required, including skin and eye irritation tests. This study aims to determine the safety of hair tonic formula of ethanol extract, ethanol fraction, and chloroform-methanol fraction from sappan wood. Ethanol extract, ethanol fraction, and chloroform-methanol fraction of sappan wood were formulated into hair tonic preparations. The hair tonic safety test includes: primary irritation test and eye irritation test using HET-CAM method. Based on the primary irritation test, the score of erythema and edema on the skin obtained a PII value of 0 (non-irritating category). The HET-CAM test on the three hair tonic formulas, positive control and negative control, showed that the positive control (lactic acid) resulted in an irritation score of 9.51 (strong irritation category); negative control (NaCl 0.9%) and the three hair tonic formulas had an irritation score of 0 (no irritation category). This indicates that the hair tonic formula from ethanol extract, ethanol fraction, and chloroform-methanol fraction of sappanwood is not irritating and safe to use.
Keywords: HET-CAM, primary irritation test, sappan wood
Downloads
Download data is not yet available.
References
Adhi Pradana, Dimas, and Bambang Hernawan Nugroho. 2016. “Uji Stabilitas Dan Uji Iritasi Primer Sediaan Kosmetik Mikroemulsi Vitamin C Palmitat (Ascorbyl Pamitate).” Jurnal Ilmiah Farmasi 12(1): 10–21.
Aditya Nugraha et al. 2020. “Acute Irritation of Tetracyclic C-4-Hydroxyphenylcalics [4] Pyrogalolarene on Skin of Albino Rabbits.” Indonesian Journal of Pharmacology and Therapy 1(1): 15–21.
Badami, Shrishailappa et al. 2003. “Antioxidant Activity of Caesalpinia Sappan Heartwood.” Biological and Pharmaceutical Bulletin 26(11): 1534–37.
Banerjee, Subham et al. 2013. “Acute Dermal Irritation, Sensitization, and Acute Toxicity Studies of a Transdermal Patch for Prophylaxis against (±) Anatoxin-a Poisoning.” International Journal of Toxicology 32(4): 308–13.
Cazedey, Edith Cristina Laignier et al. 2009. “Corrositex®, BCOP and HET-CAM as Alternative Methods to Animal Experimentation.” Brazilian Journal of Pharmaceutical Sciences 45(4): 759–66.
Cruz, Célia F. et al. 2016. “Human Hair and the Impact of Cosmetic Procedures: A Review on Cleansing and Shape-Modulating Cosmetics.” Cosmetics 3(3): 1–22.
Dewantara, I.G.N.A et al. 2015. “Uji Eritema Dan Edema Secara in Vivo Pada Natrium Lauril Sulfat 10%.” Jurnal Farmasi Udayana Volume Iv, Nomor 2 4(2): 1–21.
Dias, Maria Fernanda Reis Gavazzoni. 2015. “Hair Cosmetics: An Overview.” International Journal of Trichology 7(1): 2–15.
Febriani, Amelia, Berna Elya, and Mahdi Jufri. 2016. “Uji Aktivitas Dan Keamanan Hair Tonic Ekstrak Daun Kembang Sepatu (Hibiscus Rosa-Sinensis) Pada Pertumbuhan Rambut Kelinci.” Jurnal Farmasi Indonesia 8 No 1(1): 259–70.
Hangoluan, B. Y. M. 2011. “Pengembangan Metode Isolasi Brazilin Dari Kayu Secang (Caesalpinia Sappan).” : 24.
Harkey, M. R. 1993. “Anatomy and Physiology of Hair.” Forensic Science International 63(1–3): 9–18.
Indrawati, T., A. Syahrin, and Irpan. 2017. “Preparation of Demipermanent and Semipermanent Hair Dyes Gels from Ethanol Extract of Caesalpinia Sappan L. Using Carbomer as Gelling Agent.” AIP Conference Proceedings 1862(July).
Indriaty, Sulistiorini, Teti Indrawati, and Shelly Taurhesia. 2018. “Formulation and Test Activities of Hair Tonic with a Combination of the Aqueous Extracts of Aloe Vera ( Aloe Vera L.) and Licorice (Glycyrrhiza Glabra L.).” Pharmaciana 8(1): 33.
Luepke, NP, and FH Kemper. 1986. “The HET-CAM Test: An Alternative to the Draize Eye Test.” Food Chem Toxicol 24(6): 495–96.
Luliana, Sri, Rise Desnita, and Rani Rawinda. 2018. “Formulation of Hair Tonic of Meniran (Phyllanthus Niruri L.) Ethanol Extract as Hair Grower in Male White Rat (Rattus Norvegicus) Wistar Strain.” International Journal for Pharmaceutical Research Scholars 7(3): 136–45.
Padmaningrum, Regina Tutik, Siti Marwati, and Antuni Wiyarsi. 2012. “Karakter Ekstrak Zat Warna Kayu Secang (Caesalpinia Sappan L) Sebagai Indikator Titrasi Asam Basa.” Kimia 3(1): 1–9.
Rasouli, Hassan, Mohammad Hosein Farzaei, and Reza Khodarahmi. 2017. “Polyphenols and Their Benefits: A Review.” International Journal of Food Properties 20(00): 1700–1741. https://doi.org/10.1080/10942912.2017.1354017.
Rina, Oktaf. 2013. “Identifikasi Senyawa Aktif Dalam Ekstrak Etanol Kayu Secang ( Caesalpinia Sappan . L .).” Prosiding Semirata FMIPA Universitas Lampung: 215–18.
Rowe, Raymond c, Paul J Sheskey, and Marian E Quinn. 2009. Sixth Edit Handbook of Pharmaceutical Excipients. Sixth Edit. Washington: Pharmaceutical Press and American Pharmacists Association.
Sari dan Suhartati, Ramdana et al. 2010. “Secang (Caesalpinia Sappan L.) : Tumbuhan Herbal Kaya Antioksidan.” : 57–68.
Sari, Dina Yuspita, Ari Widiyantoro, and Andi Hairil Alimuddin. 2018. “Isolasi Brazilin Dari Kayu Secang (Caesalpinia Sappan L.) Dan Formulasinya Untuk Lipstik Batang.” Jurnal Ilmu Dan Terapan Kimia 3(1): 1–15.
Sarker, satyajit d, Zahid Latif, and alexander i Gray. 2006. Natural Products Isolation. second. eds. satyajit d Sarker, Zahid Latif, and alexander i Gray. new jersey: humana press.
Schrader, Karlheinz, and Andreas Domsch. 2005. Cosmetology-Theory and Practitice. ii. eds. Karlheinz Schrader and Andreas Domsch. augsburg: verlag fur chemische industrie.
Silva, O, A Rougier, and K. G Dossou. 1992. “The HET-CAM Test: A Study of Irritation Potential of Chemicals and Formulations (L’OREAL).” Altern. Labor. Anim 20(3): 432–37.
Toding, Lusty Grace, and A. Karim Zulkarnain. 2015. “OPTIMIZING FORMULA AND QUALITATIVE PRIMARY IRRITATION TEST IN FEMALE WHITE RABBIT OF W/O CREAM THE ETHANOLIC EXTRACT OF PHALERIA FRUIT [Phaleria Macrocarpa (Scheff.) Boerl.].” Majalah Farmaseutik 11(2): 321–27.
Utami, Sheila Meitania, Joshita Djajadisastra, and Fadlina Chany Saputri. 2017. “Using Hair Growth Activity, Physical Stability, and Safety Tests to Study Hair Tonics Containing Ethanol Extract of Licorice (Glycyrrhiza Glabra Linn.).” International Journal of Applied Pharmaceutics 9(Iii): 44–48.
Vardhani, Afifah K. 2019. “Proceedings of International Conference on Applied Science and Health ICASH-A042 Caesalpinia Sappan L : REVIEW ARTICLE Proceedings of International Conference on Applied Science and Health.” Proceedings of International Conference on Applied Science and Health (4): 300–305.
Wong, Hendra Wijaya. 2013. “RESEARCH ARTICLE EFFECTIVENESS TEST OF FENUGREEK SEED ( Trigonella Foenum-Graecum L .) EXTRACT HAIR TONIC IN HAIR GROWTH ACTIVITY * Wong Hendra Wijaya , Abdul Mun ’ Im , and Joshita Djajadisastra Faculty of Pharmacy , University of Indonesia , Depok , Ind.” International Journal of Current Research 5(1): 3453–60.
Yuliani, Sri Hartati, Yumi Rahmadani, and Enade Perdana Istyastono. 2016. “Uji Iritasi Sediaan Gel Penyembuh Luka Ekstrak Etanol Daun Binahong Menggunakan Slug Irritation Test.” Jurnal Ilmu Kefarmasian Indonesia 14(2): 135–40. http://jurnalfarmasi.or.id/index.php/jifi/article/view/22.
Aditya Nugraha et al. 2020. “Acute Irritation of Tetracyclic C-4-Hydroxyphenylcalics [4] Pyrogalolarene on Skin of Albino Rabbits.” Indonesian Journal of Pharmacology and Therapy 1(1): 15–21.
Badami, Shrishailappa et al. 2003. “Antioxidant Activity of Caesalpinia Sappan Heartwood.” Biological and Pharmaceutical Bulletin 26(11): 1534–37.
Banerjee, Subham et al. 2013. “Acute Dermal Irritation, Sensitization, and Acute Toxicity Studies of a Transdermal Patch for Prophylaxis against (±) Anatoxin-a Poisoning.” International Journal of Toxicology 32(4): 308–13.
Cazedey, Edith Cristina Laignier et al. 2009. “Corrositex®, BCOP and HET-CAM as Alternative Methods to Animal Experimentation.” Brazilian Journal of Pharmaceutical Sciences 45(4): 759–66.
Cruz, Célia F. et al. 2016. “Human Hair and the Impact of Cosmetic Procedures: A Review on Cleansing and Shape-Modulating Cosmetics.” Cosmetics 3(3): 1–22.
Dewantara, I.G.N.A et al. 2015. “Uji Eritema Dan Edema Secara in Vivo Pada Natrium Lauril Sulfat 10%.” Jurnal Farmasi Udayana Volume Iv, Nomor 2 4(2): 1–21.
Dias, Maria Fernanda Reis Gavazzoni. 2015. “Hair Cosmetics: An Overview.” International Journal of Trichology 7(1): 2–15.
Febriani, Amelia, Berna Elya, and Mahdi Jufri. 2016. “Uji Aktivitas Dan Keamanan Hair Tonic Ekstrak Daun Kembang Sepatu (Hibiscus Rosa-Sinensis) Pada Pertumbuhan Rambut Kelinci.” Jurnal Farmasi Indonesia 8 No 1(1): 259–70.
Hangoluan, B. Y. M. 2011. “Pengembangan Metode Isolasi Brazilin Dari Kayu Secang (Caesalpinia Sappan).” : 24.
Harkey, M. R. 1993. “Anatomy and Physiology of Hair.” Forensic Science International 63(1–3): 9–18.
Indrawati, T., A. Syahrin, and Irpan. 2017. “Preparation of Demipermanent and Semipermanent Hair Dyes Gels from Ethanol Extract of Caesalpinia Sappan L. Using Carbomer as Gelling Agent.” AIP Conference Proceedings 1862(July).
Indriaty, Sulistiorini, Teti Indrawati, and Shelly Taurhesia. 2018. “Formulation and Test Activities of Hair Tonic with a Combination of the Aqueous Extracts of Aloe Vera ( Aloe Vera L.) and Licorice (Glycyrrhiza Glabra L.).” Pharmaciana 8(1): 33.
Luepke, NP, and FH Kemper. 1986. “The HET-CAM Test: An Alternative to the Draize Eye Test.” Food Chem Toxicol 24(6): 495–96.
Luliana, Sri, Rise Desnita, and Rani Rawinda. 2018. “Formulation of Hair Tonic of Meniran (Phyllanthus Niruri L.) Ethanol Extract as Hair Grower in Male White Rat (Rattus Norvegicus) Wistar Strain.” International Journal for Pharmaceutical Research Scholars 7(3): 136–45.
Padmaningrum, Regina Tutik, Siti Marwati, and Antuni Wiyarsi. 2012. “Karakter Ekstrak Zat Warna Kayu Secang (Caesalpinia Sappan L) Sebagai Indikator Titrasi Asam Basa.” Kimia 3(1): 1–9.
Rasouli, Hassan, Mohammad Hosein Farzaei, and Reza Khodarahmi. 2017. “Polyphenols and Their Benefits: A Review.” International Journal of Food Properties 20(00): 1700–1741. https://doi.org/10.1080/10942912.2017.1354017.
Rina, Oktaf. 2013. “Identifikasi Senyawa Aktif Dalam Ekstrak Etanol Kayu Secang ( Caesalpinia Sappan . L .).” Prosiding Semirata FMIPA Universitas Lampung: 215–18.
Rowe, Raymond c, Paul J Sheskey, and Marian E Quinn. 2009. Sixth Edit Handbook of Pharmaceutical Excipients. Sixth Edit. Washington: Pharmaceutical Press and American Pharmacists Association.
Sari dan Suhartati, Ramdana et al. 2010. “Secang (Caesalpinia Sappan L.) : Tumbuhan Herbal Kaya Antioksidan.” : 57–68.
Sari, Dina Yuspita, Ari Widiyantoro, and Andi Hairil Alimuddin. 2018. “Isolasi Brazilin Dari Kayu Secang (Caesalpinia Sappan L.) Dan Formulasinya Untuk Lipstik Batang.” Jurnal Ilmu Dan Terapan Kimia 3(1): 1–15.
Sarker, satyajit d, Zahid Latif, and alexander i Gray. 2006. Natural Products Isolation. second. eds. satyajit d Sarker, Zahid Latif, and alexander i Gray. new jersey: humana press.
Schrader, Karlheinz, and Andreas Domsch. 2005. Cosmetology-Theory and Practitice. ii. eds. Karlheinz Schrader and Andreas Domsch. augsburg: verlag fur chemische industrie.
Silva, O, A Rougier, and K. G Dossou. 1992. “The HET-CAM Test: A Study of Irritation Potential of Chemicals and Formulations (L’OREAL).” Altern. Labor. Anim 20(3): 432–37.
Toding, Lusty Grace, and A. Karim Zulkarnain. 2015. “OPTIMIZING FORMULA AND QUALITATIVE PRIMARY IRRITATION TEST IN FEMALE WHITE RABBIT OF W/O CREAM THE ETHANOLIC EXTRACT OF PHALERIA FRUIT [Phaleria Macrocarpa (Scheff.) Boerl.].” Majalah Farmaseutik 11(2): 321–27.
Utami, Sheila Meitania, Joshita Djajadisastra, and Fadlina Chany Saputri. 2017. “Using Hair Growth Activity, Physical Stability, and Safety Tests to Study Hair Tonics Containing Ethanol Extract of Licorice (Glycyrrhiza Glabra Linn.).” International Journal of Applied Pharmaceutics 9(Iii): 44–48.
Vardhani, Afifah K. 2019. “Proceedings of International Conference on Applied Science and Health ICASH-A042 Caesalpinia Sappan L : REVIEW ARTICLE Proceedings of International Conference on Applied Science and Health.” Proceedings of International Conference on Applied Science and Health (4): 300–305.
Wong, Hendra Wijaya. 2013. “RESEARCH ARTICLE EFFECTIVENESS TEST OF FENUGREEK SEED ( Trigonella Foenum-Graecum L .) EXTRACT HAIR TONIC IN HAIR GROWTH ACTIVITY * Wong Hendra Wijaya , Abdul Mun ’ Im , and Joshita Djajadisastra Faculty of Pharmacy , University of Indonesia , Depok , Ind.” International Journal of Current Research 5(1): 3453–60.
Yuliani, Sri Hartati, Yumi Rahmadani, and Enade Perdana Istyastono. 2016. “Uji Iritasi Sediaan Gel Penyembuh Luka Ekstrak Etanol Daun Binahong Menggunakan Slug Irritation Test.” Jurnal Ilmu Kefarmasian Indonesia 14(2): 135–40. http://jurnalfarmasi.or.id/index.php/jifi/article/view/22.
Published
2021-12-31
How to Cite
SARI, Dina Yuspita; RAHMAN, Ika Ristia.
Keamanan Hair Tonic Ekstrak Etanol, Fraksi Etanol, dan Fraksi Kloroform-Metanol dari Kayu Secang (Caesalpinia sappan L.) dengan Metode Uji Iritasi Primer dan HET-CAM.
Jurnal Farmasi Udayana, [S.l.], p. 156-162, dec. 2021.
ISSN 2622-4607.
Available at: <https://ojs.unud.ac.id/index.php/jfu/article/view/76055>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.24843/JFU.2021.v10.i02.p08.
Section
Articles
