Penentuan Kadar Flavonoid Total Ekstrak Etanol Jamur Susu Harimau (Lignosus rhinocerus)
Abstract
ABSTRAK
Jamur susu harimau (Lignosus rhinocerus) merupakan tanaman yang digunakan dalam pengobatan tradisional di pedalaman Kalimantan Barat terutama di Kapuas Hulu. Salah satu konstituen fitokimia yang terkandung adalah flavonoid (flavon dan flavanon). Penelitian ini bertujuan untuk menentukan kadar flavonoid total pada ekstrak etanol jamur susu harimau. Simplisia jamur susu harimau dimaserasi menggunakan etanol 96 %. Skrining fitokima terhadap keberadaan flavonoid dalam ekstrak menggunakan serbuk magnesium dan amil alkohol. Penentuan kadar flavonoid total pada ekstrak etanol jamur susu harimau dilakukan secara spektrofotometri UV-Vis dengan metode kolorimetri (AlCl3) pada ? 410 nm dan dinyatakan sebagai flavonoid total dalam ekuivalen kuersetin (EQ). Kadar flavonoid total pada ekstrak etanol jamur susu harimau adalah sebesar 33,041 mgEQ/g ekstrak. Ekstrak etanol jamur susu harimau yang mengandung flavonoid berpotensi sebagai sumber antiooksidan alami dan potensial untuk dikembangkan dalam produk obat dan kosmetik.
Kata kunci: Jamur susu harimau, kadar flavonoid total, kolorimetri.
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References
Tan, W. C., Kuppusamy, U. R., Phan, C.W., Tan, Y.S., Raman, J., Anuar, A. M. (2015). Ganoderma neojaponicum Imazeki revisited: domestication study and antioxidant properties of its basidiocarps and mycelia. Sci. Rep. 5(12). 515. doi: 10.1038/srep12515.
Yap, H. Y., Tan, N. H, Ng, S. T., Tan, C. S, & Fung, S. Y. (2018) Inhibition of Protein Glycation by Tiger Milk Mushroom [Lignosus rhinocerus (Cooke) Ryvarden] and Search for Potential Anti-diabetic Activity-Related Metabolic Pathways by Genomic and Transcriptomic Data Mining. Front. Pharmacol. 9(103). 103-105. doi: 10.3389/fphar.2018.00103.
Johnathan, M., Gan, S. H., Ezumi, M. F. W., Faezahtul, A. H., and Nurul, A. A. (2016). Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma. BMC Complement. Alternat. Med. 16(167). 1-3. doi: 10.1186/s12906-016-1141-x.
Lee, S. Y., Kim, J. S., Kim, J. E., Sapkota, K., Shen, M. H., Kim, S. (2005). Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea. Prot. Expr. Pur. 43, 10–17. doi: 10.1016/j.pep.2005.05.004.
Hoe, T. L. (2014). Lignosus rhinocerus Attenuated High Fat Diet Induced Non-Alcoholic Fatty Liver. National Chung Hsing University.
Lee, S. S., Tan, N. H., Fung, S. Y., Tan, C. S., Ng, S. T., and Sim, S.M. (2012). Antiinflammatory activity of Lignosus rhinocerus (tiger milk mushroom) sclerotia. in Abstracts of the 18th Congress of the International Society for Mushroom Science (Beijing), 150–151.
Mohanarji, S. Dharmalingam, S., Kalusalingam, A. 2012. Screening of Lignosus rhinocerus Extracts as Antimicrobial Agents Against Selected Human Pathogens. Journal of Pharmaceutical and Biomedical Sciences (JPBMS). 18(11). 1-3.
Abdullah, N., Wahab, A. I. A., Lau, B. F., Abidin, N. Z., & Aminudin, N. (2010). Anti-cervical cancer activity and SELDI-TOF-MS analysis of proteins from Lignosus rhinocerus (Tiger’s Milk Mushroom) grown in stirred tank reactor. Proceedings of the Fifth International Peptide Symposium.
Marzouk, M. M. 2016. Flavonoid Constituents And Cytotoxic Activity Of Erucaria Hispanica (L.) Druce Growing Wild In Egypt. Arabian Journal Of Chemistry. 9(1). 411–415.
Wong, K. H., and Cheung, P. C. K. (2009). Sclerotia: emerging functional food derived from mushrooms in Mushrooms as Functional Foods, ed P. C. K. Cheung (111-146). Hoboken, NJ: JohnWiley & Sons, Inc.
Hasmi, N. A. H., Nordin, N., Kumar, Y., Lani, N., Fazil, F. N. M., Aizad, S., Abidin, N. Z., Zubairi, S. I. 2016. Analysis of Flavonoids in Commercially Available Lignosus Rhinocerus Dried Powder. Extract by Reversed-Phase High Performance Liquid Cromatograph (RP-HPLC). International Future Scientis Conference. Universiti Kebangsaan Malaysia (UKM).
Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. 2019. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Jurnal Plants., 8(96). 12-13. doi:10.3390/plants8040096.
Azizah, D. N., Kumolowati, E., Faramayuda, F. 2014. Penetapan Kadar Flavonoid Metode AlCl3 Pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi. 2(2). 47.
Parthasarathi, S., and Park, Y. K., 2015. Determination of total phenolics, flavonoid contents and antioxidant activity of different mBHT fractions: A polyherbal medicine. Pakistan journal of pharmaceutical sciences. 28(6). 2162-2164.
Rebaya, A., Belghith, S. I., Baghdikian, B., Leddet, V. M., Mabrouki, F., Olivier, E.,, Cherif, J. K., Ayadi, M. T. 2014. Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae). Journal of Applied Pharmaceutical Science. 5(1). 52-57. doi: 10.7324/JAPS.2015.50110.
Ipandi, I., Triyasmono, L., dan Prayitno, B. 2016. Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leucosyke capitellata Wedd). Pharmascience, 3(1), 93-100.
Syafitri, N. S., Bintang, M., Falah, S. 2014. Kandungan Fitokimia, Total Fenol, dan Total Flavonoid Ekstrak Buah Harendong (Melastoma affine D. Don). Current Biochemistry. 1(3). 105-115.
Sugrani, A., Wagner, H. 2009. Plant Drug Analysis, a Thin Layer Chromatography Atlas, second Edition, 1-365. Springer.
Ahmad, A. R., Juwita, Ratulangi, S. A. D., dan Malik, A. 2015. Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Pharm Sci Res. 2(1). 1-10.
Nallathambya, N., Serma, L. G., Raman, J., Maleka, S. N. A., Vidyadarana, S.,, Naidua, M., Kuppusamya, U. R., & Sabaratnama, V. 2016. Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities. Natural Product Communication. 11(10). 1485-1490.
Suhendi, A., Sjahid, L. A., Hanwar, D. 2011. Isolasi dan Identifikasi Flavonoid Dari Daun Dewandaru (Eugenia uniflora L.). Pharmacon, 12(2).
Yap, H. Y., Tan, N. H, Ng, S. T., Tan, C. S, & Fung, S. Y. (2018) Inhibition of Protein Glycation by Tiger Milk Mushroom [Lignosus rhinocerus (Cooke) Ryvarden] and Search for Potential Anti-diabetic Activity-Related Metabolic Pathways by Genomic and Transcriptomic Data Mining. Front. Pharmacol. 9(103). 103-105. doi: 10.3389/fphar.2018.00103.
Johnathan, M., Gan, S. H., Ezumi, M. F. W., Faezahtul, A. H., and Nurul, A. A. (2016). Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma. BMC Complement. Alternat. Med. 16(167). 1-3. doi: 10.1186/s12906-016-1141-x.
Lee, S. Y., Kim, J. S., Kim, J. E., Sapkota, K., Shen, M. H., Kim, S. (2005). Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea. Prot. Expr. Pur. 43, 10–17. doi: 10.1016/j.pep.2005.05.004.
Hoe, T. L. (2014). Lignosus rhinocerus Attenuated High Fat Diet Induced Non-Alcoholic Fatty Liver. National Chung Hsing University.
Lee, S. S., Tan, N. H., Fung, S. Y., Tan, C. S., Ng, S. T., and Sim, S.M. (2012). Antiinflammatory activity of Lignosus rhinocerus (tiger milk mushroom) sclerotia. in Abstracts of the 18th Congress of the International Society for Mushroom Science (Beijing), 150–151.
Mohanarji, S. Dharmalingam, S., Kalusalingam, A. 2012. Screening of Lignosus rhinocerus Extracts as Antimicrobial Agents Against Selected Human Pathogens. Journal of Pharmaceutical and Biomedical Sciences (JPBMS). 18(11). 1-3.
Abdullah, N., Wahab, A. I. A., Lau, B. F., Abidin, N. Z., & Aminudin, N. (2010). Anti-cervical cancer activity and SELDI-TOF-MS analysis of proteins from Lignosus rhinocerus (Tiger’s Milk Mushroom) grown in stirred tank reactor. Proceedings of the Fifth International Peptide Symposium.
Marzouk, M. M. 2016. Flavonoid Constituents And Cytotoxic Activity Of Erucaria Hispanica (L.) Druce Growing Wild In Egypt. Arabian Journal Of Chemistry. 9(1). 411–415.
Wong, K. H., and Cheung, P. C. K. (2009). Sclerotia: emerging functional food derived from mushrooms in Mushrooms as Functional Foods, ed P. C. K. Cheung (111-146). Hoboken, NJ: JohnWiley & Sons, Inc.
Hasmi, N. A. H., Nordin, N., Kumar, Y., Lani, N., Fazil, F. N. M., Aizad, S., Abidin, N. Z., Zubairi, S. I. 2016. Analysis of Flavonoids in Commercially Available Lignosus Rhinocerus Dried Powder. Extract by Reversed-Phase High Performance Liquid Cromatograph (RP-HPLC). International Future Scientis Conference. Universiti Kebangsaan Malaysia (UKM).
Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. 2019. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Jurnal Plants., 8(96). 12-13. doi:10.3390/plants8040096.
Azizah, D. N., Kumolowati, E., Faramayuda, F. 2014. Penetapan Kadar Flavonoid Metode AlCl3 Pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi. 2(2). 47.
Parthasarathi, S., and Park, Y. K., 2015. Determination of total phenolics, flavonoid contents and antioxidant activity of different mBHT fractions: A polyherbal medicine. Pakistan journal of pharmaceutical sciences. 28(6). 2162-2164.
Rebaya, A., Belghith, S. I., Baghdikian, B., Leddet, V. M., Mabrouki, F., Olivier, E.,, Cherif, J. K., Ayadi, M. T. 2014. Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae). Journal of Applied Pharmaceutical Science. 5(1). 52-57. doi: 10.7324/JAPS.2015.50110.
Ipandi, I., Triyasmono, L., dan Prayitno, B. 2016. Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leucosyke capitellata Wedd). Pharmascience, 3(1), 93-100.
Syafitri, N. S., Bintang, M., Falah, S. 2014. Kandungan Fitokimia, Total Fenol, dan Total Flavonoid Ekstrak Buah Harendong (Melastoma affine D. Don). Current Biochemistry. 1(3). 105-115.
Sugrani, A., Wagner, H. 2009. Plant Drug Analysis, a Thin Layer Chromatography Atlas, second Edition, 1-365. Springer.
Ahmad, A. R., Juwita, Ratulangi, S. A. D., dan Malik, A. 2015. Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Pharm Sci Res. 2(1). 1-10.
Nallathambya, N., Serma, L. G., Raman, J., Maleka, S. N. A., Vidyadarana, S.,, Naidua, M., Kuppusamya, U. R., & Sabaratnama, V. 2016. Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities. Natural Product Communication. 11(10). 1485-1490.
Suhendi, A., Sjahid, L. A., Hanwar, D. 2011. Isolasi dan Identifikasi Flavonoid Dari Daun Dewandaru (Eugenia uniflora L.). Pharmacon, 12(2).
Published
2021-07-01
How to Cite
SARI, Dina Yuspita; R, Widyasari; AN, Taslima.
Penentuan Kadar Flavonoid Total Ekstrak Etanol Jamur Susu Harimau (Lignosus rhinocerus).
Jurnal Farmasi Udayana, [S.l.], p. 23-30, july 2021.
ISSN 2622-4607.
Available at: <https://ojs.unud.ac.id/index.php/jfu/article/view/66543>. Date accessed: 05 nov. 2024.
doi: https://doi.org/10.24843/JFU.2021.v10.i01.p03.
Section
Articles
