ANALISIS 8-ISOPROSTAN DALAM URIN TIKUS JANTAN WISTAR SETELAH TERPAPAR ETANOL DAN ASAP ROKOK

  • Agung Ari Chandra Wibawa
  • Ni Made Suaniti
  • Ni Komang Ariati

Abstract

The 8-isoprostane compound can be used for a biomarker of oxidative stress  formed by lipid peroxidation in the form of arachidonic acid with free radical in the body. The purpose of this study was to analysis  urinary 8-isoprostane in male Wistar rats after sub-acute exposer to ethanol and cigarette smoke. Extraction method used was solid phase extraction and the analysis was conducted by TLC and spectrophotodensitometry. The results showed that the 8-isoprostane compound was not detected in the male Wistar rat’s urine as control rats and in those exposed to cigarette smoke. The average concentrations of 8-Isoprostane in the male Wistar rat’s urine exposed to ethanol and combination of ethanol-cigarette were 1.519 ± 1,386 and 2.063 ± 1,308 ppm respectively.

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Author Biographies

Agung Ari Chandra Wibawa
Jurusan Kimia FMIPA Universitas Udayana, Kampus Bukit Jimbaran
Ni Made Suaniti
Jurusan Kimia FMIPA Universitas Udayana, Kampus Bukit Jimbaran
Ni Komang Ariati
Jurusan Kimia FMIPA Universitas Udayana, Kampus Bukit Jimbaran
Published
2014-01-01
How to Cite
ARI CHANDRA WIBAWA, Agung; SUANITI, Ni Made; ARIATI, Ni Komang. ANALISIS 8-ISOPROSTAN DALAM URIN TIKUS JANTAN WISTAR SETELAH TERPAPAR ETANOL DAN ASAP ROKOK. Jurnal Kimia (Journal of Chemistry), [S.l.], jan. 2014. ISSN 2599-2740. Available at: <https://ojs.unud.ac.id/index.php/jchem/article/view/9747>. Date accessed: 07 apr. 2020.
Section
Articles

Keywords

8-isoprostane, Arachidonic acid, TLC, Spectrophotodensitometry