Phytochemical Analysis of Bulung Boni (Caulerpa cylindracea S.) N- Hexane Extract with Gc-Ms Method and Toxicity Test on Mice (Mus musculus L.)
Abstract
One of the marine resource commodities owned by Indonesia is seaweed. Bulung boni is one type of seaweed that grown in Indonesia. Bulung boni is the regional name of Caulerpa cylindracea. Bulung boni is usually used as a food and medicine for most people in Bali. The purpose of this research is to study the content of phytochemical compounds in n-hexane extract of bulung boni and determine the toxicity effect on mice (Mus musculus). The methods used in this study were maceration, GC-MS (Gas Chromatography-Mass Spectrometry), observation, and calculation with the Thomson and Weil formula. This study used 24 mice as experimental animals. Mice were divided into 6 treatment groups, group 1 as control was given 1% Na-CMC solution, groups 2-6 were given test extracts with doses, 100, 200, 300, 400, 500 mg/30 g BW mice. The results showed the highest compound contained in the n-hexane extract of bulung boni was Hexadecanoic acid, methyl ester with an AUC value of 35.21%. Toxicity tests on mice showed no deaths experienced by mice, so the LD50 value determined was pseudo at 16.6 g/ kg BW and fell into the practically non-toxic category
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References
Abubacker, M. N., & Deepalakshmi, T. (2013). In vitro Antifungal Potentials of Bioactive Compound Methyl Ester of Hexadecanoic Acid Isolated from Annona muricata Linn. (Annonaceae) Leaves. Biosciences Biotechnology Research Asia Vol. 10(2), 879-884.
Armansyah, T. T. R., Sudi, I., Amalia, S., Rosmaidar, R., Nuzul, A., Budianto, P., Dwinna, A., Hamdan, H. (2016). Uji Toksisitas Akut Ekstrak Etanol Daun Malaka (Phyllantus emblica) Terhadap Mencit (Mus musculus). Banda Aceh: Fakultas Kedokteran Universitas Syiah Kuala.
BPOM. (2014). Pedoman Uji Toksisitas Nonklinik Secara In Vivo. Badan Pengawas Obat dan Makanan Republik Indonesia.
Budiana. (2008). Memahami Dampak Kolesterol. Available from: URL: http:// www.dewansfamilymultiply.com.
Hanani, E., Mun’im, A., & Sekarini, R. (2005). Identifikasi Senyawa Antioksidan dalam Spons Callyspongia sp. dari Kepulauan Seribu. Majalah Ilmiah Kefarmasian, 3(2): 127-133.
Jenova, R. (2009). Uji Toksisitas Akut yang Diukur Dengan Penentuan LD50 Ekstrak Herba Putri Malu (Mimosa pudica l.) Terhadap Mencit balb/c. Laporan Akhir Penelitian Karya Tulis Ilmiah. Fakultas Kedokteran Universitas Diponegoro. Semarang.
Julyasih, K.S.M., Wirawan, I.G.P., Harijani, W.S., dan Widajati, W. (2009). Aktivitas Antioksidan beberapa Jenis Rumput Laut (Seaweeds) Komersial di Bali. Seminar Nasional ‘Akselerasi Pengembangan Teknologi Pertanian dalam Mendukung Revitalisasi Pertanian’. Fakultas Pertanian & Lppm UPN Veteran. Jawa Timur.
Kim, D. H., Min, H. P., Yeon, J. C., Ki, W.C., Chan, H. P., Eun, J. J., Hye, J.A., Byung, P. Y., & Hae, Y. C. (2013). Molecular Study of Dietary Heptadecane for the Anti- Inflammatory Modulation of NF-kB in the Aged Kidney . Department of Pharmacy, College of Pharmacy, Aging Tissue Bank, Pusan National University, Gumjung-gu, Busan, Republic of Korea.
Loomis, T. A., Lea., and Febiger. (1987). Essential of Toxicology. Journal of Pharmaceutical Sciences. 3rd ed. 600 Washington Square. Philadelpia.
Lu, F. C., & Kacew, S. (2002). ‘Coventional Toxicity Studies’ in Lu, F.C. and Kacew, S., Basic Toxicology: Fundamentals, Target Organs, and Risk Assessment, 4th edition, Taylor and Francis, London and New York, pp 77.
Manikandan, G., Vimala R. A., Divya, C and Ramasubbu, R. (2017). GC-MS Analysis Of Phytochemical Constituents In The Petroleum Ether Leaf Extracts Of Millettia peguensis,” vol. 8, no. 9, pp. 144–150.
Mishra, S., Verma, S. S., Rai, V., Awasthee, N., Arya, J. S., Maiti, K. K., & Gupta, S. C. (2019). Curcuma raktakanda Induces Apoptosis and Suppresses Migration in Cancer Cells: Role of Reactive Oxygen Species. Biomolecules 9(4): 159.
Nazzi, F., Norberto, M., Giorgio, D. V. (2002). (Z)-8-Heptadecene from Infested Cells Reduces the Reproduction of Varroa destructor Under Laboratory Conditions. Journal of Chemical Ecology, Vol. 28, No. 11. Italy.
PubChem. (2004). Hexadecane. https://pubchem.ncbi.nlm.nih.gov/compound/ 11006
PubChem. (2004). Nonadecane. https://pubchem.ncbi.nlm.nih.gov/compound/ 12401
PubChem. (2004). Tetradecane. https://pubchem.ncbi.nlm.nih.gov/c ompound/ 12389
PubChem. (2005). 2,4-Di-tert-butylphenol. https://pubchem.ncbi.nlm.nih.gov/ compound/7311
PubChem. (2005). Hexadecanoic acid, ethyl ester. https://pubchem.ncbi.nlm. nih.gov/compound/12366
PubChem. (2005). Methyl tetradecanoate. https://pubchem.ncbi.nlm.nih.gov/ compound/31284
PubChem. (2005). Phenol, 2 methoxy-. https://pubchem.ncbi.nlm.nih.gov/ compound/460
Rodrigues, M. J., Custódio, L., Lopes, A., Oliveira, M., Neng, N. R., Nogueira, J. M., Martins, A., Rauter, A. P., Varela, P., & Barreira, L. (2017). Unlocking the in vitro anti-inflammatory and antidiabetic potential of Polygonum maritimum. Pharmaceutical biology 55(1): 1348-1357.
Siems, W., Quast, S., Carluccio, F., Wiswedel, I., Hirsch, D., Augustin, W., Hampi, H., Riehle, M., Sommerburg, O. (2002). Oxidative Stress in Chronic Renal Failure as A Cardiovascular Risk Factor. Clin. Nephrol. 58 (Suppl 1), S12–S19 (Jul).
Subroto, M. A. (2008). Real Food, True Health. Makanan Sehat Untuk Hidup Lebih Sehat. PT AgroMedia Pustaka, Jakarta.
Trono, J. R. G . C., & Ganzon, E. T. (1988). Philippine Seaweed. Publ. by National Book Store. Inc : 327 pp.
Wahyuni, D. T., & Widjanarko, S. B. (2015). Pengaruh Jenis Pelarut dan Lama Ekstraksi terhadap Ekstrak Karotenoid Labu Kuning dengan Metode Gelombang Ultrasonik. Jurnal Pangan dan Agroindustri Vol. 3 No 2 P.390-401.
Armansyah, T. T. R., Sudi, I., Amalia, S., Rosmaidar, R., Nuzul, A., Budianto, P., Dwinna, A., Hamdan, H. (2016). Uji Toksisitas Akut Ekstrak Etanol Daun Malaka (Phyllantus emblica) Terhadap Mencit (Mus musculus). Banda Aceh: Fakultas Kedokteran Universitas Syiah Kuala.
BPOM. (2014). Pedoman Uji Toksisitas Nonklinik Secara In Vivo. Badan Pengawas Obat dan Makanan Republik Indonesia.
Budiana. (2008). Memahami Dampak Kolesterol. Available from: URL: http:// www.dewansfamilymultiply.com.
Hanani, E., Mun’im, A., & Sekarini, R. (2005). Identifikasi Senyawa Antioksidan dalam Spons Callyspongia sp. dari Kepulauan Seribu. Majalah Ilmiah Kefarmasian, 3(2): 127-133.
Jenova, R. (2009). Uji Toksisitas Akut yang Diukur Dengan Penentuan LD50 Ekstrak Herba Putri Malu (Mimosa pudica l.) Terhadap Mencit balb/c. Laporan Akhir Penelitian Karya Tulis Ilmiah. Fakultas Kedokteran Universitas Diponegoro. Semarang.
Julyasih, K.S.M., Wirawan, I.G.P., Harijani, W.S., dan Widajati, W. (2009). Aktivitas Antioksidan beberapa Jenis Rumput Laut (Seaweeds) Komersial di Bali. Seminar Nasional ‘Akselerasi Pengembangan Teknologi Pertanian dalam Mendukung Revitalisasi Pertanian’. Fakultas Pertanian & Lppm UPN Veteran. Jawa Timur.
Kim, D. H., Min, H. P., Yeon, J. C., Ki, W.C., Chan, H. P., Eun, J. J., Hye, J.A., Byung, P. Y., & Hae, Y. C. (2013). Molecular Study of Dietary Heptadecane for the Anti- Inflammatory Modulation of NF-kB in the Aged Kidney . Department of Pharmacy, College of Pharmacy, Aging Tissue Bank, Pusan National University, Gumjung-gu, Busan, Republic of Korea.
Loomis, T. A., Lea., and Febiger. (1987). Essential of Toxicology. Journal of Pharmaceutical Sciences. 3rd ed. 600 Washington Square. Philadelpia.
Lu, F. C., & Kacew, S. (2002). ‘Coventional Toxicity Studies’ in Lu, F.C. and Kacew, S., Basic Toxicology: Fundamentals, Target Organs, and Risk Assessment, 4th edition, Taylor and Francis, London and New York, pp 77.
Manikandan, G., Vimala R. A., Divya, C and Ramasubbu, R. (2017). GC-MS Analysis Of Phytochemical Constituents In The Petroleum Ether Leaf Extracts Of Millettia peguensis,” vol. 8, no. 9, pp. 144–150.
Mishra, S., Verma, S. S., Rai, V., Awasthee, N., Arya, J. S., Maiti, K. K., & Gupta, S. C. (2019). Curcuma raktakanda Induces Apoptosis and Suppresses Migration in Cancer Cells: Role of Reactive Oxygen Species. Biomolecules 9(4): 159.
Nazzi, F., Norberto, M., Giorgio, D. V. (2002). (Z)-8-Heptadecene from Infested Cells Reduces the Reproduction of Varroa destructor Under Laboratory Conditions. Journal of Chemical Ecology, Vol. 28, No. 11. Italy.
PubChem. (2004). Hexadecane. https://pubchem.ncbi.nlm.nih.gov/compound/ 11006
PubChem. (2004). Nonadecane. https://pubchem.ncbi.nlm.nih.gov/compound/ 12401
PubChem. (2004). Tetradecane. https://pubchem.ncbi.nlm.nih.gov/c ompound/ 12389
PubChem. (2005). 2,4-Di-tert-butylphenol. https://pubchem.ncbi.nlm.nih.gov/ compound/7311
PubChem. (2005). Hexadecanoic acid, ethyl ester. https://pubchem.ncbi.nlm. nih.gov/compound/12366
PubChem. (2005). Methyl tetradecanoate. https://pubchem.ncbi.nlm.nih.gov/ compound/31284
PubChem. (2005). Phenol, 2 methoxy-. https://pubchem.ncbi.nlm.nih.gov/ compound/460
Rodrigues, M. J., Custódio, L., Lopes, A., Oliveira, M., Neng, N. R., Nogueira, J. M., Martins, A., Rauter, A. P., Varela, P., & Barreira, L. (2017). Unlocking the in vitro anti-inflammatory and antidiabetic potential of Polygonum maritimum. Pharmaceutical biology 55(1): 1348-1357.
Siems, W., Quast, S., Carluccio, F., Wiswedel, I., Hirsch, D., Augustin, W., Hampi, H., Riehle, M., Sommerburg, O. (2002). Oxidative Stress in Chronic Renal Failure as A Cardiovascular Risk Factor. Clin. Nephrol. 58 (Suppl 1), S12–S19 (Jul).
Subroto, M. A. (2008). Real Food, True Health. Makanan Sehat Untuk Hidup Lebih Sehat. PT AgroMedia Pustaka, Jakarta.
Trono, J. R. G . C., & Ganzon, E. T. (1988). Philippine Seaweed. Publ. by National Book Store. Inc : 327 pp.
Wahyuni, D. T., & Widjanarko, S. B. (2015). Pengaruh Jenis Pelarut dan Lama Ekstraksi terhadap Ekstrak Karotenoid Labu Kuning dengan Metode Gelombang Ultrasonik. Jurnal Pangan dan Agroindustri Vol. 3 No 2 P.390-401.
Published
2023-04-21
How to Cite
SILALAHI, May Disa Br et al.
Phytochemical Analysis of Bulung Boni (Caulerpa cylindracea S.) N- Hexane Extract with Gc-Ms Method and Toxicity Test on Mice (Mus musculus L.).
International Journal of Biosciences and Biotechnology, [S.l.], v. 10, n. 2, p. 41 - 51, apr. 2023.
ISSN 2655-9994.
Available at: <https://ojs.unud.ac.id/index.php/jbb/article/view/105932>. Date accessed: 08 july 2024.
doi: https://doi.org/10.24843/IJBB.2023.v10.i02.p06.
Section
Articles
